並列タイトル等大腸亜全摘術後のラットにおいてアクアポリン8の発現は増強する
Enhanced aquaporin 8 expression after subtotal colectomy in rat.
一般注記Background: Aquaporins (AQPs), the family of water-selective channels, are localized in various organs and tissues, including the gastrointestinal (GI) tract. However, the roles of AQPs in the GI tract remain unclear. Materials and Methods: Male SD rats were subjected to subtotal colectomy (Group C, n = 22) or a sham operation (Group S, n = 16) and were sacrificed on postoperative days 7, 14, and 28. Total RNAs from the distal ileum and rectum were extracted. Quantitative RT-PCR was performed to measure AQP8 mRNA expression. For light-microscopy or immunohistochemistry, paraffin-embedded sections of 4 μm were prepared with H-E staining or anti-AQP8 antibody reaction. Mann-Whitney U-test was performed to compare the AQP8 distributions between the two groups, and the statistical significance was defined as p < 0.05. Results: AQP8 mRNA expression was enhanced in both the ileum and rectum in Group C at day 7. AQP8 protein expression was consistently observed in the ileum and rectum. The villus length in the ileum of Group C was significantly greater than that of Group S at days 7 and 14. Conclusion: Enhanced AQP8 mRNA expression in the subtotal colectomy model suggests that AQP8 plays an important role in maintaining the intestinal fluid balance.
学位の種類: 博士（医学）. 報告番号: 乙第2200号. 学位記番号: 新大博（医）乙第1782号. 学位授与年月日: 平成28年3月23日
Open Journal of Gastroenterology. 2013, 3(5), 253-258.
新大博（医）乙第1782号
元資料の権利情報 : Copyright(C) 2013 Masato Nakano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOIinfo:doi/10.4236/ojgas.2013.35043
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