Optimization of mNeonGreen for Homo sapiens increases its fluorescent intensity in mammalian cells

• 資料種別: 記事
• タイトル: Optimization of mNeonGreen for Homo sapiens increases its fluorescent intensity in mammalian cells
• 著者標目: Emiko Tanida-Miyake; Masato Koike; Yasuo Uchiyama; Isei Tanida; https://orcid.org/0000-0001-8999-3990
• 出版年月日等: 2018-01-17
• 出版年（W3CDTF）: 2018-01-17
• 寄与者: Makoto Sato
• タイトル（掲載誌）: PLOS ONE
• 巻号年月日等（掲載誌）: 13 1
• 掲載巻: 13
• 掲載号: 1
• 掲載ページ: e0191108-
• 掲載年月日（W3CDTF）: 2018-01-17
• 出版事項（掲載誌）: Public Library of Science (PLoS)
• 件名標目: Science; Q; Green Fluorescent Proteins; R; DNA; Mitochondria; HEK293 Cells; COS Cells; Chlorocebus aethiops; Medicine; Animals; Humans; Codon; Coloring Agents; Research Article; Plasmids
• 対象利用者: 一般
• 標準番号（その他）: PMID : 29342181
• DOI: 10.1371/journal.pone.0191108
• 作成日（W3CDTF）: 2018-01-17
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: http://creativecommons.org/licenses/by/4.0/
• 関連情報（URI）: http://dx.plos.org/10.1371/journal.pone.0191108
• 参照: Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins; https://cir.nii.ac.jp/crid/1360009142829139840; Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein; https://cir.nii.ac.jp/crid/1360009142833122560; In-resin CLEM of Epon-embedded cells using proximity labeling; https://cir.nii.ac.jp/crid/1360017280666204544
• 参照: Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity; https://cir.nii.ac.jp/crid/1360004233538600576; Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells; https://cir.nii.ac.jp/crid/1360011145005846016; The Bright Fluorescent Protein mNeonGreen Facilitates Protein Expression Analysis<i>In Vivo</i>; https://cir.nii.ac.jp/crid/1360011145279229440; A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites; https://cir.nii.ac.jp/crid/1360011145337001088; Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells; https://cir.nii.ac.jp/crid/1360574093706261888; Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system; https://cir.nii.ac.jp/crid/1360574095215386240; A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum; https://cir.nii.ac.jp/crid/1361137045041383296; FACS-optimized mutants of the green fluorescent protein (GFP); https://cir.nii.ac.jp/crid/1361699995031308032; Quantitative assessment of fluorescent proteins; https://cir.nii.ac.jp/crid/1361699995510235008; Development of fluorescence expression tools to study host-mycoplasma interactions and validation in two distant mycoplasma clades; https://cir.nii.ac.jp/crid/1363951793864317056; Multiple Epitope Tagging of Expressed Proteins for Enhanced Detection; https://cir.nii.ac.jp/crid/1363951795001752960; Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins; https://cir.nii.ac.jp/crid/1364233270650230272
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Crossref; https://www.crossref.org; 科学研究費助成事業データベース; https://kaken.nii.ac.jp/ja

• 要約等: Green fluorescent protein (GFP) is tremendously useful for investigating many cellular and intracellular events. The monomeric GFP mNeonGreen is about...