The common ancestral core of vertebrate and fungal telomerase RNAs

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Author: Qi, Xiaodongほか

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Publication date: 2013-01

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Note (General)：: 出版タイプ： AOTelomerase is a ribonucleoprotein with an intrinsic telomerase RNA (TER) component. Within yeasts, TER is remarkably large and presents littl...

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Material Type: その他
Title: The common ancestral core of vertebrate and fungal telomerase RNAs
Author/Editor: Qi, Xiaodong
Li, Yang
Honda, Shinji
Hoffmann, Steve
Marz, Manja
Mosig, Axel
Podlevsky, Joshua D.
Stadler, Peter F.
Selker, Eric U.
Chen, Julian J.-L.
Author Heading: Qi, Xiaodong
Li, Yang
Honda, Shinji
Hoffmann, Steve
Marz, Manja
Mosig, Axel
Podlevsky, Joshua D.
Stadler, Peter F.
Selker, Eric U.
Chen, Julian J.-L.
Publication Date: 2013-01
Publication Date (W3CDTF): 2013
Periodical title: Nucleic Acids Research
No. or year of volume/issue: 41 1
Volume: 41
Issue: 1
Pages: 450-462
Target Audience: 一般
Note (General): 出版タイプ： AO
Telomerase is a ribonucleoprotein with an intrinsic telomerase RNA (TER) component. Within yeasts, TER is remarkably large and presents little similarity in secondary structure to vertebrate or ciliate TERs. To better understand the evolution of fungal telomerase, we identified 74 TERs from Pezizomycotina and Taphrinomycotina subphyla, sister clades to budding yeasts. We initially identified TER from Neurospora crassa using a novel deep-sequencing-based approach, and homologous TER sequences from available fungal genome databases by computational searches. Remarkably, TERs from these non-yeast fungi have many attributes in common with vertebrate TERs. Comparative phylogenetic analysis of highly conserved regions within Pezizomycotina TERs revealed two core domains nearly identical in secondary structure to the pseudoknot and CR4/5 within vertebrate TERs. We then analyzed N. crassa and Schizosaccharomyces pombe telomerase reconstituted in vitro, and showed that the two RNA core domains in both systems can reconstitute activity in trans as two separate RNA fragments. Furthermore, the primer-extension pulse-chase analysis affirmed that the reconstituted N. crassa telomerase synthesizes TTAGGG repeats with high processivity, a common attribute of vertebrate telomerase. Overall, this study reveals the common ancestral cores of vertebrate and fungal TERs, and provides insights into the molecular evolution of fungal TER structure and function.
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ