Effects of Ovarian Theca Cells on Granulosa Cell Differentiation During Gonadotropin-Independent Follicular Growth in Cattle

• Material Type: 文書・図像類
• Title: Effects of Ovarian Theca Cells on Granulosa Cell Differentiation During Gonadotropin-Independent Follicular Growth in Cattle
• Author/Editor: Orisaka, Makoto; Mizutani, Tetsuya; Tajima, Kimihisa; Orisaka, Sanae; Shukunami, Ken-ichi; Miyamoto, Kaoru; Kotsuji, Fumikazu
• Author Heading: Orisaka, Makoto; Mizutani, Tetsuya; Tajima, Kimihisa; Orisaka, Sanae; Shukunami, Ken-ichi; Miyamoto, Kaoru; Kotsuji, Fumikazu
• Publication, Distribution, etc.: John Wiley & Sons
• Publication Date: 2006-03
• Publication Date (W3CDTF): 2006-03
• Periodical title: Molecular Reproduction and Development
• No. or year of volume/issue: 73 6
• Volume: 73
• Issue: 6
• Pages: 737-744
• ISSN (Periodical Title): ISSN : 1040-452X
• Text Language Code: eng
• Subject Heading: aromatase; estrogen; follicle; granulosa cells; theca cells
• Target Audience: 一般
• Note (General): We investigated the effects of theca cells or FSH on granulosa cell differentiation and steroid production during bovine early follicular growth, using a co-culture system in which granulosa and theca cells were cultured on opposite sides of a collagen membrane. Follicular cells were isolated from early antral follicles (2-4 mm) that were assumed to be in gonadotropin-independent phase and just before recruitment into a follicular wave. Granulosa cells were cultured under serum-free conditions with and without theca cells or recombinant human FSH to test their effects on granulosa cell differentiation. Messenger RNA levels for P450 aromatase (aromatase), P450 cholesterol side chain cleavage (P450scc), 3-hydroxysteroid dehydrogenase (3-HSD), LH receptor (LHr), and steroidogenic acute regulatory protein (StAR) in granulosa cells were measured by real-time quantitative RT-PCR analysis. FSH enhanced aromatase mRNA expression in granulosa cells, but did not alter estradiol production. FSH also enhanced mRNA expression for P450scc, LHr, and StAR in granulosa cells, resulting in an increase in progesterone production. In contrast, theca cells enhanced aromatase mRNA expression in granulosa cells resulting in an increase in estradiol production. Theca cells did not alter progesterone production and mRNA expression in granulosa cells for P450scc, 3-HSD, LHr, and StAR. The results of the present study indicate that theca cells are involved in both rate-limiting steps in estrogen production, i.e., androgen substrate production and aromatase regulation, and that theca cell-derived factors regulate estradiol and progesterone production in a way that reflects steroidogenesis during the follicular phase of the estrous cycle.
• Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=20565&item_no=1&attribute_id=22&file_no=1
• Access Restrictions: 限定公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ