The Virion Proteins Encoded by Bacteriophage ΦK and Its Host-Range Mutant ΦKhT: Host-Range Determination and DNA Binding Properties1

Material type: 文書・図像類

Author: KODAIRA, Ken-Ichiほか

Publisher: The Japanese Biochemical Society

Publication date: 1996

Material Format: Digital

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Note (General)：: The microvirid phage ΦK, specific for Escherichia coli K12, contains a circular single-stranded (SS) DNA in the icosahedral virion, which comprises fo...

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Material Type: 文書・図像類
Title: The Virion Proteins Encoded by Bacteriophage ΦK and Its Host-Range Mutant ΦKhT: Host-Range Determination and DNA Binding Properties1
Author/Editor: KODAIRA, Ken-Ichi
OKI, Masaya
KAKIKAWA, Makiko
KIMOTO, Hisashi
TAKETO, Akira
Author Heading: KODAIRA, Ken-Ichi
OKI, Masaya
KAKIKAWA, Makiko
KIMOTO, Hisashi
TAKETO, Akira
Publication, Distribution, etc.: The Japanese Biochemical Society
Publication Date: 1996
Publication Date (W3CDTF): 1996
Periodical title: The Journal of Biochemistry
No. or year of volume/issue: 119 6
Volume: 119
Issue: 6
Pages: 1062-1069
ISSN (Periodical Title): ISSN : 0021-924X
Text Language Code: eng
Subject Heading: bacteriophage
DNA-binding protein
host-range
ΦK
virion proteins
Target Audience: 一般
Note (General): The microvirid phage ΦK, specific for Escherichia coli K12, contains a circular single-stranded (SS) DNA in the icosahedral virion, which comprises four phage gene products, F (capsid), G (major spike), H (minor spike), and J (core). ΦKhT, a host-range mutant of ΦK, can grow on E. coli C and B, besides K12, and is more thermosensitive than the parental phage ΦK. Sequencing analysis revealed that the genome of ΦK and ΦKhT consists of 6,089 nucleotides (nt), and codes for eleven genes, whose sequences are similar to those of α3, ΦX174, and G4 infective to strain C. In ΦKhT, two nt had changed: one is in the gene G, resulting in replacement of the 75th codon Ala with Ser, and the other is at 67th codon of the gene H: Val to Ala. Chemically synthesized gene J protein composed of 23 amino acids (aa) binds to ΦK SS DNA more tightly than and preferentially over the host E. coli SS-DNA-binding protein (SSB). These results indicate that the two spike proteins G and H are involved in the determination of ΦK host-range, and support a model in which the gene J protein functions in packaging the viral SS DNA into the virion vesicle.
Access Restrictions: 限定公開
Rights (production): 〓 1996 The Japanese Biochemical Society
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ