アルカリ性ピクリン酸法及び高速液体クロマトグラフィによる尿中クレアチニンの定量

Material type: 文書・図像類

Author: 戎, 利光ほか

Publisher: 日本教育医学研究会

Publication date: 1986-08

Material Format: Digital

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Note (General)：: Urinary creatinine has been widely analyzed in research fields concerning body composition and muscular activities since the amount of creatinine excr...

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Ｕｎｉｖｅｒｓｉｔｙ　ｏｆ　Ｆｕｋｕｉ　Ａｃａｄｅｍｉｃ　Ｒｅｐｏｓｉｔｏｒｙ
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Material Type: 文書・図像類
Title: アルカリ性ピクリン酸法及び高速液体クロマトグラフィによる尿中クレアチニンの定量
Author/Editor: 戎, 利光
目, 不二雄
菅原, 泰一
EBISU, Toshimitsu
SAKAN, Fujio
SUGAHARA, Taiichi
Author Heading: 戎, 利光
目, 不二雄
菅原, 泰一
EBISU, Toshimitsu
SAKAN, Fujio
SUGAHARA, Taiichi
Publication, Distribution, etc.: 日本教育医学研究会
Publication Date: 1986-08
Publication Date (W3CDTF): 1986-08
Alternative Title: DETERMINING URINARY CREATININE BY ALKALINE PICRATE METHOD AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Periodical title: 教育医学
No. or year of volume/issue: 32 1・2
Volume: 32
Issue: 1・2
Pages: 89-94
Text Language Code: jpn
Target Audience: 一般
Note (General): Urinary creatinine has been widely analyzed in research fields concerning body composition and muscular activities since the amount of creatinine excretion in urine is nearly proportional to the muscular mass in humans, and the amount in an individual is nearly constant. And also research studies pointed out the decrease in urinary creatinine after acute exercise. Effects of exercise upon urinary creatinine have been, furthermore, reported. However, there are research studies indicating that Borsook method shows slightly lower values than Folin method and that the liquid chromatographic procedure gives results that are lower than those obtained by an alkaline picrate method(APM) and the procedure is more precise. The purpose of this study is, therefore, to clarify the preciseness of APM in a case of determining urinary creatinine. Thirty one healthy college male students volunteered to be subjects in this study. Urinary creatinine values by APM was compared with those by a high performance liquid chromatography(HPLC). A Folin-Wu method was used for APM. This study found that urinary creatinine values by HPLC is significantly lower than those by APM as Patel and George indicated although there was a significant correlation between the values by HPLC and APM. And also it was found that a significant regression formula, y=1.0168x-118.099(where x=urinary creatinine concentration by APM),should be used to obtain more precise creatinine concentration in urine which is y values, urinary creatinine concentration by HPLC, if HPLC is not available.
Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=23276&item_no=1&attribute_id=22&file_no=1
https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=23276&item_no=1&attribute_id=22&file_no=1
Access Restrictions: 限定公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ