Tumor uptake of radiolabeled acetate reflects the expression of cytosolic acetyl-CoA synthetase: implications for the mechanism of acetate PET

• Material Type: 文書・図像類
• Title: Tumor uptake of radiolabeled acetate reflects the expression of cytosolic acetyl-CoA synthetase: implications for the mechanism of acetate PET
• Author/Editor: YOSHII, Yukie; WAKI, Atsuo; FURUKAWA, Takako; KIYONO, Yasushi; MORI, Tetsuya; YOSHII, Hiroshi; KUDO, Takashi; OKAZAWA, Hidehiko; WELCH, Michael J.; FUJIBAYASHI, Yasuhisa
• Author Heading: YOSHII, Yukie; WAKI, Atsuo; FURUKAWA, Takako; KIYONO, Yasushi; MORI, Tetsuya; YOSHII, Hiroshi; KUDO, Takashi; OKAZAWA, Hidehiko; WELCH, Michael J.; FUJIBAYASHI, Yasuhisa
• Publication, Distribution, etc.: ELSEVIER B.V.
• Publication Date: 2009-10
• Publication Date (W3CDTF): 2009-10
• Periodical title: Nuclear Medicine and Biology
• No. or year of volume/issue: 36 7
• Volume: 36
• Issue: 7
• Pages: 771-777
• Text Language Code: eng
• Subject Heading: Acetate; Acetyl-CoA synthetase; PET; Hypoxia; Tumor
• Target Audience: 一般
• Note (General): Introduction: [1-^<11>C]acetate positron emission tomography (PET) is used for myocardial studies. In the myocardium, mitochondrial acetyl-CoA synthetase (ACSS1) mainly contributes to the radiopharmaceutical uptake. [1-^<11>C]acetate PET is also used for tumor diagnosis; however, the uptake mechanism of radiolabeled acetate in tumors remains unclear. Our previous study reported that cytosolic acetyl-CoA synthetase (ACSS2) was expressed in tumor cells and up-regulated under hypoxia; whereas, expression of ACSS1 was negligible regardless of the oxygen conditions. We also indicated that ACSS2 is a bi-directional enzyme that controls acetyl-CoA / acetate metabolism in tumor cells. In this study, to elucidate the basic mechanism of tumor acetate uptake, we focused on ACSS2 and investigated the role of ACSS2 in the uptake of radiolabeled acetate in tumor cells. Methods: [1-^<14>C]acetate uptake and ACSS2 expression were examined in four tumor cell lines under normoxia or hypoxia. An ACSS2 knockdown study was also performed. Results: [1-^<14>C]acetate uptake was increased in the tumor cells under hypoxia. This pattern followed that of ACSS2 expression. The incorporated ^<14>C was mostly distributed in the lipid-soluble fractions, and this tendency increased under hypoxia. ACSS2 knock down led to a corresponding reduction in [1-^<14>C]acetate uptake in all tumor cell lines examined under normoxia and hypoxia. Conclusions: ACSS2 plays an important role in the uptake of radiolabeled acetate in tumor cells, which is different from that in the myocardium, which mainly involves ACSS1. The uptake of radiolabeled acetate in tumors increased under hypoxia along with up-regulation of ACSS2 expression. This suggests a possible mechanism for acetate PET for tumors.
• Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=19776&item_no=1&attribute_id=22&file_no=1
• Access Restrictions: 限定公開
• Related Material (DOI): 10.1016/j.nucmedbio.2009.05.006
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ