Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis

• Material Type: 文書・図像類
• Title: Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis
• Author/Editor: KUBO, E.; SINGH, D. P.; AKAGI, Y.
• Author Heading: KUBO, E.; SINGH, D. P.; AKAGI, Y.
• Publication, Distribution, etc.: Springer
• Publication Date: 2005-04
• Publication Date (W3CDTF): 2005-04
• Periodical title: Diabetologia
• No. or year of volume/issue: 48 4
• Volume: 48
• Issue: 4
• Pages: 790-798
• ISSN (Periodical Title): ISSN : 0012-186X
• Text Language Code: eng
• Subject Heading: cDNA microarray; Diabetic cataract; Galactose cataract; Oxidative stress; Polyol pathway
• Target Audience: 一般
• Note (General): Aims/hypothesis Osmotic and oxidative stress is associated with the progression and advancement of diabetic cataract. In the present study, we used a cDNA microarray method to analyse gene expression patterns in streptozotocin-induced diabetic rats and galactose-fed cataractous lenses. In addition, we investigated the regulation and interaction(s) of anti-oxidant protein 2 and lens epithelium-derived growth factor in these models. Methods To identify differential gene expression patterns, one group of Sprague–Dawley rats was made diabetic with streptozotocin and a second group was made galactosaemic. Total RNA was extracted from the lenses of both groups and their controls. Labelled cDNA was hybridised to Atlas Rat Arrays. Changes in gene expression level were analysed. Real-time PCR and western analysis were used to validate the microarray results. Results The expression of 31 genes was significantly modulated in hyperglycaemic lenses compared with galactosaemic lenses. Notably, transcript and protein levels of B-cell leukaemia/lymphoma protein 2 and nuclear factor-B were significantly elevated in rat lenses at 4 weeks after injection of streptozotocin. At a later stage, mRNA and protein levels of TGF- were elevated. However, levels of mRNA for IGF-1, lens epithelium-derived growth factor and anti-oxidant protein 2 were diminished in streptozotocin-induced diabetic cataract. Conclusions/interpretations These results provide evidence that progression of sugar cataract involves oxidative- and TGF--mediated signalling. These pathways may promote abnormal gene expression in the hyperglycaemic and galactosaemic states and thus may contribute to the symptoms associated with these conditions. Since oxidative stress seems to be a major event in cataract formation, supply of anti-oxidant may postpone the progression of such disorders.
• Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=20526&item_no=1&attribute_id=22&file_no=1
• Access Restrictions: 限定公開
• Related Material (DOI): 10.1007/s00125-005-1687-5
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ