Determination of the single strand origin of Shigella sonnei plasmid pKYM

Material type: 文書・図像類

Author: KODAIRA, Ken-Ichiほか

Publisher: Elsevier Science B.V.

Publication date: 1995-01

Material Format: Digital

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Note (General)：: The Shigella sonnei plasmid pKYM replicates by a rolling-circle mechanism in Escherichia coli. A 571 nucleotides HincII restriction fragment of the pK...

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Material Type: 文書・図像類
Title: Determination of the single strand origin of Shigella sonnei plasmid pKYM
Author/Editor: KODAIRA, Ken-Ichi
OKI, Masaya
TAKETO, Akira
YASUKAWA, Hiroo
MASAMUNE, Yukito
Author Heading: KODAIRA, Ken-Ichi
OKI, Masaya
TAKETO, Akira
YASUKAWA, Hiroo
MASAMUNE, Yukito
Publication, Distribution, etc.: Elsevier Science B.V.
Publication Date: 1995-01
Publication Date (W3CDTF): 1995-01
Periodical title: Biochimica et Biophysica Acta
No. or year of volume/issue: 1260 2
Volume: 1260
Issue: 2
Pages: 183-190
Text Language Code: eng
Subject Heading: Single-stranded DNA plasmid
Origin mutant
Deletion
Complementary strand
DNA sequence
Target Audience: 一般
Note (General): The Shigella sonnei plasmid pKYM replicates by a rolling-circle mechanism in Escherichia coli. A 571 nucleotides HincII restriction fragment of the pKYM DNA harbors two potential hairpin loops (I and II). We cloned the fragment into a -ori defective M13 vector phage, M13 delta lac183. The chimera phage, MDKY5, showed a larger plaque size, and increased phage yield and rate of progeny replicative form DNA (RF) synthesis. Rifampicin reduced rate of conversion of the single- to double-stranded RF DNA. In addition, we introduced nucleotide deletions within the cloned pKYM DNA, by Bal31 nuclease digestion. Each of the deletion mutants thus constructed was lacking in a sequence containing the hairpin loops and formed smaller plaques. The in vivo analyses revealed that a 136 nucleotides sequence containing the two hairpins I and II is the pKYM minus origin for complementary strand synthesis (single strand origin, referred to as SSO) and harbors a recognition site(s) by host E. coli RNA polymerase, for primer RNA synthesis. Moreover, we found a 24 nt sequence, upstream of the SSO domain having 83% homology to the recombination site A (RSA) which functions in plasmid sitespecific recombination and/or transfer.
Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=22278&item_no=1&attribute_id=22&file_no=1
https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=22278&item_no=1&attribute_id=22&file_no=1
Access Restrictions: 限定公開
Related Material (DOI): 10.1016/0167-4781(94)00199-D
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ