Promoter/repressor system of Lactobacillus plantarum phage øg1e: characterization of the promoters pR49–pR–pL and overproduction of the Cro-like protein Cng in Escherichia coli

Material type: 文書・図像類

Author: KAKIKAWA, Makikoほか

Publisher: Elsevier Science B.V.

Publication date: 1998-07

Material Format: Digital

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Note (General)：: The Lactobacillus plantarum phage øg1e (42 259 bp) has two repressor-like genes cng and cpg oriented oppositely, accompanied by three potential promot...

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Material Type: 文書・図像類
Title: Promoter/repressor system of Lactobacillus plantarum phage øg1e: characterization of the promoters pR49–pR–pL and overproduction of the Cro-like protein Cng in Escherichia coli
Author/Editor: KAKIKAWA, Makiko
WATANABE, Nobukatsu
FUNAWATASHI, Tatsuya
OKI, Masaya
YASUKAWA, Hiroo
TAKETO, Akira
KODAIRA, Ken-Ichi
Author Heading: KAKIKAWA, Makiko
WATANABE, Nobukatsu
FUNAWATASHI, Tatsuya
OKI, Masaya
YASUKAWA, Hiroo
TAKETO, Akira
KODAIRA, Ken-Ichi
Publication, Distribution, etc.: Elsevier Science B.V.
Publication Date: 1998-07
Publication Date (W3CDTF): 1998-07
Periodical title: Gene -Amsterdam-
No. or year of volume/issue: 215 2
Volume: 215
Issue: 2
Pages: 371-379
Text Language Code: eng
Subject Heading: DNA-binding protein
Gel-shift assay
Operator
Primer extension
Target Audience: 一般
Note (General): The Lactobacillus plantarum phage øg1e (42 259 bp) has two repressor-like genes cng and cpg oriented oppositely, accompanied by three potential promoters pR, pL and pR49, and seven operator-like sequences (GATAC-boxes) (Kodaira et al., 1997). In this study, the øg1e putative promoters were introduced into the Escherichia coli promoter-detecting plasmid pKK232-8. In E. coli CK111, pR (pKPR1), pL (pKPL1) and pR49 (pKPR49) exhibited distinct CAT activities. When pKPR1 or pKPL1 was coexistent with a compatible plasmid pACYC184 carrying pR-cng (pA4PRCN1), the CAT activity was decreased significantly. On the other hand, cng directed a protein (Cng) of 10.1 kDa in E. coli under the control of T7 promoter. Gel mobility-shift assays demonstrated that Cng binds specifically to a DNA region containing the GATAC-boxes. In addition, primer extension analyses demonstrated that the two sequences pR and pL act as a promoter in L. plantarum as well as in E. coli. These results suggested that the potential promoters pR and pL probably function for the lytic and lysogenic pathways, respectively, and Cng may act as a repressor presumably through the GATAC-boxes as operators.
Access Restrictions: 限定公開
Related Material (DOI): 10.1016/S0378-1119(98)00289-3
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ