Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

Material type: 文書・図像類

Author: TAKAHASHI, Toruほか

Publisher: 日本分析化学会

Publication date: 2013-11

Material Format: Digital

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Note (General)：: A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous so...

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Material Type: 文書・図像類
Title: Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium
Author/Editor: TAKAHASHI, Toru
KAWANA, Jun
TAMURA, Yuuki
HOSHINO, Hitoshi
Author Heading: TAKAHASHI, Toru
KAWANA, Jun
TAMURA, Yuuki
HOSHINO, Hitoshi
Publication, Distribution, etc.: 日本分析化学会
Publication Date: 2013-11
Publication Date (W3CDTF): 2013-11
Periodical title: Analytical Sciences
No. or year of volume/issue: 29 11
Volume: 29
Issue: 11
Pages: 1099-1102
Text Language Code: eng
Subject Heading: Capillary electrophoresis
dynamic coating
hydroxyethyl cellulose
polyethylene glycol
humic acid
DNA ladder
Target Audience: 一般
Note (General): A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous solution containing hydroxyethyl cellulose (HEC), polyethylene glycol (PEG), and pH buffer component was employed for both the dynamic coating agent and the separation medium. The procedure for the coating of the capillary inner wall was simply filling the buffered polymer mixture solution into the capillary that had been treated with 1 M aqueous HC1 solution. The solution for the capillary coating was directly used as the electrophoretic buffer solution for CE separation. Excellent performance for the separation of humic acid was obtained using the solution containing 0.5% (w/v) HEC, 1.0% (w/v) PEG 10000, and 0.1% (w/v) PEG 8000000. Excellent reproducibility and durability were obtained even at slightly alkaline conditions at pH levels above 8. The separation of 0.1 - 2 kbp of DNA ladder by the proposed method showed was also achieved.
Access Restrictions: 限定公開
Rights (production): c 2103 The Japan Society for Analytical Chemistry
Related Material (DOI): 10.2116/analsci.29.1099
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ