Growth Differentiation Factor 9 Is Antiapoptotic during Follicular Development from Preantral to Early Antral Stage

• Material Type: 文書・図像類
• Title: Growth Differentiation Factor 9 Is Antiapoptotic during Follicular Development from Preantral to Early Antral Stage
• Author/Editor: ORISAKA, Makoto; ORISAKA, Sanae; Jiang, Jin-Yi; Craig, Jesse; WANG, Yifang; KOTSUJI, Fumikazu; Tsang, Benjamin K.
• Author Heading: ORISAKA, Makoto; ORISAKA, Sanae; Jiang, Jin-Yi; Craig, Jesse; WANG, Yifang; KOTSUJI, Fumikazu; Tsang, Benjamin K.
• Publication, Distribution, etc.: The Endocrine Society
• Publication Date: 2006-05
• Publication Date (W3CDTF): 2006-05
• Periodical title: Molecular Endocrinology
• No. or year of volume/issue: 20 10
• Volume: 20
• Issue: 10
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Ovarian follicular atresia represents a selection process that ensures the release of only healthy and viable oocytes during ovulation. The transition from preantral to early antral stage is the penultimate stage of development in terms of gonadotropin dependence and follicle destiny (survival/growth vs. atresia). We have examined whether and how oocyte-derived growth differentiation factor 9 (GDF-9) and FSH regulate follicular development and atresia during the preantral to early antral transition, by a novel combination of in vitro gene manipulation (i.e. intraoocyte injection of GDF-9 antisense oligos) and preantral follicle culture. Injection of GDF-9 antisense suppressed basal and FSH-induced preantral follicle growth in vitro, whereas addition of GDF-9 enhanced basal and FSH-induced follicular development. GDF-9 antisense activated caspase-3 and induced apoptosis in cultured preantral follicles, a response attenuated by exogenous GDF-9. GDF-9 increased phospho-Akt content in granulosa cells of early antral follicles. Although granulosa cell apoptosis induced by ceramide was attenuated by the presence of GDF-9, this protective effect of GDF-9 was prevented by the phosphatidylinositol 3-kinase inhibitor LY294002 and a dominant negative form of Akt. Injection of GDF-9 antisense decreased FSH receptor mRNA levels in cultured follicles, a response preventable by the presence of exogenous GDF-9. The data suggest that GDF-9 is antiapoptotic in preantral follicles and protects granulosa cells from undergoing apoptosis via activation of the phosphatidylinositol 3-kinase/Akt pathway. An adequate level of GDF-9 is required for follicular FSH receptor mRNA expression. GDF-9 promotes follicular survival and growth during the preantral to early antral transition by suppressing granulosa cell apoptosis and follicular atresia.
• Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=20569&item_no=1&attribute_id=22&file_no=1
• Access Restrictions: 限定公開
• Related Material (DOI): 10.1210/me.2005-0357
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ