Gene expression profiles of 1-(4-amino-2-methyl-5-pyrimidinyl)-methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU)-resistant C6 rat glioma cells

Material type: 文書・図像類

Author: NAKAGAWA, Takaoほか

Publisher: Springer

Publication date: 2006-09

Material Format: Digital

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Note (General)：: Chemotherapy in itself is suspected to cause the development or selection of drug-resistant tumor cells, which have more aggressive phenotypes. The au...

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Material Type: 文書・図像類
Title: Gene expression profiles of 1-(4-amino-2-methyl-5-pyrimidinyl)-methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU)-resistant C6 rat glioma cells
Author/Editor: NAKAGAWA, Takao
KUBOTA, Toshihiko
IDO, Kazunori
SAKUMA, Takahiro
MASUDA, Ken
Author Heading: NAKAGAWA, Takao
KUBOTA, Toshihiko
IDO, Kazunori
SAKUMA, Takahiro
MASUDA, Ken
Publication, Distribution, etc.: Springer
Publication Date: 2006-09
Publication Date (W3CDTF): 2006-09
Periodical title: Journal of neuro-oncology
No. or year of volume/issue: 79 3
Volume: 79
Issue: 3
Pages: 271-279
ISSN (Periodical Title): ISSN : 0167-594X
Text Language Code: eng
Subject Heading: Glioma
ACNU
Drug resistance
cDNA array
Glial marker
Target Audience: 一般
Note (General): Chemotherapy in itself is suspected to cause the development or selection of drug-resistant tumor cells, which have more aggressive phenotypes. The authors investigated the differential changes of gene expression in the 1 -(4-amino-2-methyl-5-pyrimidinyl)-methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU)-resistant subline of the C6 rat glioma (C6AR2), which was established from C6 rat glioma cells by exposure to ACNU in vitro. The resistance to ACNU of C6AR2 was confirmed by MTS assay. The increased expression of O^6-methylguanine-DNA methyl-transferase in C6AR2 cells was shown using RT-PCR. C6AR2 cells displayed a higher proliferative activity relative to C6 cells. Analysis with cDNA array showed that 19 genes were transcriptionally up-regulated and 16 genes down-regulated in C6AR2 cells compared to C6 cells. They belonged to various functional classes of genes beside the drug-resistant system. Among them, the down-regulation of several genes in C6AR2 cells, including c-kit, pleiotrophin, platelet-derived growth factor receptor-a, peripheral myelin protein-22 and NG2 chondroitin sulfate proteoglycan, which are expressed originally in developmental glial linages, were verified using semi-quantitative RT-PCR. In addition, the gene expression of astroglial intermediate filament proteins, including GFAP, vimentin and nestin, were decreased in C6AR2 cells relative to C6 cells in semi-quantitative RT-PCR and immunocytochemistry. These findings may represent an undifferentiated state of ACNU-resistant glioma cells and a more aggressive phenotype in recurrent tumors following chemotherapy.
Access Restrictions: 限定公開
Related Material (DOI): 10.1007/s11060-006-9143-z
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ