Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions

Material type: 文書・図像類

Author: Eguchi, Kohgakほか

Publisher: Frontiers Media

Publication date: 2020-03-19

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Note (General)：: Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain...

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Material Type: 文書・図像類
Title: Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
Author/Editor: Eguchi, Kohgak
Velicky, Philipp
Hollergschwandtner, Elena
Itakura, Makoto
Fukazawa, Yugo
Danzl, Johann Georg
Shigemoto, Ryuichi
Author Heading: Eguchi, Kohgak
Velicky, Philipp
Hollergschwandtner, Elena
Itakura, Makoto
Fukazawa, Yugo
Danzl, Johann Georg
Shigemoto, Ryuichi
Publication, Distribution, etc.: Frontiers Media
Publication Date: 2020-03-19
Publication Date (W3CDTF): 2020-03-19
Periodical title: Frontiers in Cellular Neuroscience
No. or year of volume/issue: 14
Volume: 14
Pages: 1-17
ISSN (Periodical Title): ISSN : 1662-5102
Text Language Code: eng
Subject Heading: acute brain slices
synaptic transmission
synaptic plasticity
cerebellum
super-resolution microscopy
Target Audience: 一般
Note (General): Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions.
Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=24888&item_no=1&attribute_id=22&file_no=1
https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=24888&item_no=1&attribute_id=22&file_no=1
Access Restrictions: 限定公開
Rights (production): This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Related Material (DOI): 10.3389/fncel.2020.00063
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ