Study on Irradiation Effect of Mid-Infrared Free Electron Laser on Hen Egg-White Lysozyme by Using Terahertz-Time Domain Spectroscopy and Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectroscopy

• Material Type: 文書・図像類
• Title: Study on Irradiation Effect of Mid-Infrared Free Electron Laser on Hen Egg-White Lysozyme by Using Terahertz-Time Domain Spectroscopy and Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectroscopy
• Author/Editor: Kawasaki, Takayasu; Izumi, Yudai; Ohori, Gaku; Kitahara, Hideaki; Furuya, Takashi; Yamamoto, Kohji; Matsuo, Koichi; Tani, Masahiko; Tsukiyama, Koichi
• Author Heading: Kawasaki, Takayasu; Izumi, Yudai; Ohori, Gaku; Kitahara, Hideaki; Furuya, Takashi; Yamamoto, Kohji; Matsuo, Koichi; Tani, Masahiko; Tsukiyama, Koichi
• Publication, Distribution, etc.: Springer
• Publication Date: 2019-10-15
• Publication Date (W3CDTF): 2019-10-15
• Alternative Title: Study on Irradiation Effect of Mid-Infrared Free Electron Laser on Hen Egg-White Lysozyme by Using Terahertz-Time Domain Spectroscopy and Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectroscopy
• Periodical title: Journal of Infrared, Millimeter, and Terahertz Waves
• No. or year of volume/issue: 40 10
• Volume: 40
• Issue: 10
• Pages: 998-1009
• ISSN (Periodical Title): ISSN : 1866-6892
• Text Language Code: eng
• Subject Heading: Mid-infrared free-electron laser; Protein aggregate; Terahertz-time domain spectroscopy; Vacuum-ultraviolet circular-dichroism spectroscopy
• Target Audience: 一般
• Note (General): Mid-infrared free-electron laser (MIR-FEL) is potentially applicable for tissue ablation and dissociation of pathological peptide aggregates in medicine. However, it is still poorly understood how the MIR-FEL irradiation influences on functional proteins such as enzymes. In the current study, the effect of MIR-FEL on both aggregate and non-aggregate (= native) states of hen egg-white lysozyme (HEWL) as a representative enzyme has been investigated. Absorption intensity at terahertz region (0.3–1.2 THz, 10–40 cm−1) of the aggregate o fHEWL was lower than that of the native HEWL, but the former was increased nearly to the same level with the latter after the MIR-FEL irradiation tuned to 6 μm that corresponds to carbonyl stretching vibrational mode of amide bonds (amide I). This indicates that the aggregate of HEWL was converted to the native state by the irradiation. On the other hand, synchrotronradiation vacuum-ultraviolet circular-dichroism spectroscopy showed that protein conformation of the native HEWL, which was rich in α-helix, was little changed after the MIR-FEL irradiation under the same condition with the case of the aggregate of HEWL. Furthermore, the enzymatic hydrolysis activity of the native HEWL against bacterial glycan was not remarkably decreased by the irradiation. Therefore, it can be estimated that the native structure of HEWL is little damaged although the aggregate state can be easily dissociated by the resonant excitation at amide bonds.
• Access Restrictions: 限定公開
• Rights (production): copyright 2019 Springer Science+Business Media, LLC, part of Springer Nature
• Related Material (DOI): 10.1007/s10762-019-00626-9
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ