Concurrent mass spectrometric analysis of multiple samples using Py-Tag reagents

Material type: 文書・図像類

Author: Tanaka, Yukieほか

Publisher: Elsevier BV

Publication date: 2018-11

Material Format: Digital

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Note (General)：: The quantification of amino acids, peptides and proteins are important in fundamental science to understand intravital event, as well as in applied fi...

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Material Type: 文書・図像類
Title: Concurrent mass spectrometric analysis of multiple samples using Py-Tag reagents
Author/Editor: Tanaka, Yukie
Ikeda, Akari
Matsukawa, Shigeru
Imase, Yuka
Saigyo, Miki
Maruyama, Chitose
Fujieda, Shigeharu
Taira, Shu
Author Heading: Tanaka, Yukie
Ikeda, Akari
Matsukawa, Shigeru
Imase, Yuka
Saigyo, Miki
Maruyama, Chitose
Fujieda, Shigeharu
Taira, Shu
Publication, Distribution, etc.: Elsevier BV
Publication Date: 2018-11
Publication Date (W3CDTF): 2018-11
Alternative Title: Concurrent mass spectrometric analysis of multiple samples using Py-Tag reagents
Periodical title: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY = INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
No. or year of volume/issue: 434
Volume: 434
Pages: 158-163
ISSN (Periodical Title): ISSN : 1387-3806
Text Language Code: eng
Subject Heading: Proteomics
Py-Tag
LC–MS
Stable isotope
Target Audience: 一般
Note (General): The quantification of amino acids, peptides and proteins are important in fundamental science to understand intravital event, as well as in applied fields such as biological, medical, and pharmaceutical sciences.Py-Tags (Py0, Py6 and Py12) are primary amine-reactive isotope-labelled tagging reagents. Py-Tags can selectively and efficiently react with the primary amino group which has more than a two-carbon chain from the alpha-carbon of the target sample by the reaction of pyrylium salts with nucleophiles under alkaline conditions, thereby increasing the mass by 175.15, 181.17, and 187.19 Da, respectively, and generating positive ions.We used Py-Tags to label not only the amino acids but also peptides with a lysine residue(s), fragment peptides with a lysine(s), digested proteins, and intact proteins. All Py-Tag-labelled peptide sets showed similar mass differences. Thus, we could easily analyze expression ratio by single measurement. Furthermore, the ratio of the measured ion intensities was representative of the relative amount of each material.
source:https://www.sciencedirect.com/science/article/abs/pii/S1387380618301945
Source: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=28958&item_no=1&attribute_id=22&file_no=1
https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=28958&item_no=1&attribute_id=22&file_no=1
Access Restrictions: 限定公開
Related Material (DOI): 10.1016/j.ijms.2018.09.007
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 福井大学 : 福井大学学術機関リポジトリ