Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm

• Material Type: 博士論文
• Title: Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm
• Author/Editor: Asikin, Nur; Hirota, Katsuhiko; Yumoto, Hiromichi; Hirao, Kouji; Liu, Dali; Takahashi, Kanako; Murakami, Keiji; Matsuo, Takashi; Shu, Rong; Miyake, Yoichiro
• Author Heading: Asikin, Nur; Hirota, Katsuhiko; Yumoto, Hiromichi; Hirao, Kouji; Liu, Dali; Takahashi, Kanako; Murakami, Keiji; Matsuo, Takashi; Shu, Rong; Miyake, Yoichiro
• Publication, Distribution, etc.: The Society for Applied Microbiology|John Wiley & Sons Inc
• Publication Date: 2013-03-29
• Publication Date (W3CDTF): 2013-03-29
• Alternative Title: Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響; Effect of eDNA on biofilm formation
• Periodical title: Journal of Applied Microbiology
• No. or year of volume/issue: 115 1
• Volume: 115
• Issue: 1
• Pages: 260-270
• ISSN (Periodical Title): ISSN : 1365-2672; ISSN : 1364-5072
• Degree grantor/type: 徳島大学
• Date Granted: 2014-09-10
• Dissertation Number: 甲第2757号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: extracellular DNA; extracellular HLP; biofilm development; Streptococcus intermedius; DNA concentration
• Target Audience: 一般
• Note (General): Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity. Methods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains. Conclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity. Significance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
• Format (IMT): application/pdf
• Source: fulltext; http://repo.lib.tokushima-u.ac.jp/files/public/10/106279/20170929145117945265/LID201409191007.pdf; http://repo.lib.tokushima-u.ac.jp/files/public/10/106279/20170929145118281255/LID201409191008.pdf; http://repo.lib.tokushima-u.ac.jp/files/public/10/106279/20191008172917273100/k2757_fulltext.pdf
• Access Restrictions: インターネット公開
• Rights (production): This is the peer reviewed version of the following article: Nur, A., Hirota, K., Yumoto, H., Hirao, K., Liu, D., Takahashi, K., Murakami, K., Matsuo, T., Shu, R. and Miyake, Y. (2013), Effects of extracellular DNA and DNA‐binding protein on the development of a Streptococcus intermedius biofilm. J Appl Microbiol, 115: 260-270., which has been published in final form at https://doi.org/10.1111/jam.12202. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• Related Material (DOI): 10.1111/jam.12202
• Periodical Title (NCID): AA11115564; https://ci.nii.ac.jp/ncid/AA11115564
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ