Hypertrophic Chondrocytes in the Rabbit Growth Plate Can Proliferate and Differentiate into Osteogenic Cells when Capillary Invasion Is Interposed by a Membrane Filter

• Material Type: 博士論文
• Title: Hypertrophic Chondrocytes in the Rabbit Growth Plate Can Proliferate and Differentiate into Osteogenic Cells when Capillary Invasion Is Interposed by a Membrane Filter
• Author/Editor: Enishi, Tetsuya; Yukata, Kiminori; Takahashi, Mitsuhiko; Sato, Ryosuke; Sairyo, Koichi; Yasui, Natsuo
• Author Heading: Enishi, Tetsuya; Yukata, Kiminori; Takahashi, Mitsuhiko; Sato, Ryosuke; Sairyo, Koichi; Yasui, Natsuo
• Publication, Distribution, etc.: PLOS
• Publication Date: 2014-08-14
• Publication Date (W3CDTF): 2014-08-14
• Alternative Title: 成長軟骨板において膜フィルターで血管侵入を阻害すると肥大軟骨細胞は増殖し骨芽細胞様細胞に分化する。; Hypertrophic Chondrocytes Differentiate into Osteogenic Cells
• Periodical title: PLOS ONE
• No. or year of volume/issue: 9 8
• Volume: 9
• Issue: 8
• Pages: e104638-
• ISSN (Periodical Title): ISSN : 1932-6203
• Degree grantor/type: 徳島大学
• Date Granted: 2014-10-23
• Dissertation Number: 甲第2778号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: 軟骨細胞; 成長軟骨版; 軟骨内骨化; 骨芽細胞; 分化
• Target Audience: 一般
• Note (General): The fate of hypertrophic chondrocytes during endochondral ossification remains controversial. It has long been thought that the calcified cartilage is invaded by blood vessels and that new bone is deposited on the surface of the eroded cartilage by newly arrived cells. The present study was designed to determine whether hypertrophic chondrocytes were destined to die or could survive to participate in new bone formation. In a rabbit experiment, a membrane filter with a pore size of 1 mm was inserted in the middle of the hypertrophic zone of the distal growth plate of ulna. In 33 of 37 animals, vascular invasion was successfully interposed by the membrane filter. During 8 days, the cartilage growth plate was enlarged, making the thickness 3-fold greater than that of the nonoperated control side. Histological examination demonstrated that the hypertrophic zone was exclusively elongated. At the terminal end of the growth plate, hypertrophic chondrocytes extruded from their territorial matrix into the open cavity on the surface of the membrane filter. The progenies of hypertrophic chondrocytes (PHCs) were PCNA positive and caspase-3 negative. In situ hybridization studies demonstrated that PHCs did not express cartilage matrix proteins anymore but expressed bone matrix proteins. Immunohistochemical studies also emonstrated that the new matrix produced by PHCs contained type I collagen, osteonectin, and osteocalcin. Based on these results, we concluded that hypertrophic chondrocytes switched into bone-forming cells after vascular invasion was interposed in the normal growth plate.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): Copyright: © 2014 Enishi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ( https://creativecommons.org/licenses/by/4.0/ )
• Related Material (DOI): 10.1371/journal.pone.0104638
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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