Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly

• Material Type: 博士論文
• Title: Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly
• Author/Editor: Murota, Yukiko; Ishizu, Hirotsugu; Nakagawa, Shinichi; Iwasaki, Yuka W.; Shibata, Shinsuke; Kamatani, Miharu K.; Saito, Kuniaki; Okano, Hideyuki; Shiomi, Haruhiko; Siomi, Mikiko C.
• Author Heading: Murota, Yukiko; Ishizu, Hirotsugu; Nakagawa, Shinichi; Iwasaki, Yuka W.; Shibata, Shinsuke; Kamatani, Miharu K.; Saito, Kuniaki; Okano, Hideyuki; Shiomi, Haruhiko; Siomi, Mikiko C.
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2014-06-19
• Publication Date (W3CDTF): 2014-06-19
• Alternative Title: YbはpiRNA前駆体とpiRNA生合成因子を核周辺部位に形成される顆粒体に局在させることによってpiRISC形成を促進する
• Periodical title: Cell Reports
• No. or year of volume/issue: 8 1
• Volume: 8
• Issue: 1
• Pages: 103-113
• ISSN (Periodical Title): ISSN : 2211-1247
• Degree grantor/type: 徳島大学
• Date Granted: 2014-10-23
• Dissertation Number: 甲第2779号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: piRNA; Piwi; flamenco; Yb body; Drosophila
• Target Audience: 一般
• Note (General): PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable for formation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
• Related Material (DOI): 10.1016/j.celrep.2014.05.043
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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