Effect of Light Irradiation by Light Emitting Diode on Colon Cancer Cells

• Material Type: 博士論文
• Title: Effect of Light Irradiation by Light Emitting Diode on Colon Cancer Cells
• Author/Editor: Matsumoto, Noriko; Yoshikawa, Kozo; Shimada, Mitsuo; Kurita, Nobuhiro; Sato, Hirohiko; Iwata, Takashi; Higashijima, Jun; Chikakiyo, Motoya; Nishi, Masaaki; Kashihara, Hideya; Takasu, Chie; Eto, Shohei; Takahashi, Akira; Akutagawa, Masatake; Emoto, Takahiro
• Author Heading: Matsumoto, Noriko; Yoshikawa, Kozo; Shimada, Mitsuo; Kurita, Nobuhiro; Sato, Hirohiko; Iwata, Takashi; Higashijima, Jun; Chikakiyo, Motoya; Nishi, Masaaki; Kashihara, Hideya; Takasu, Chie; Eto, Shohei; Takahashi, Akira; Akutagawa, Masatake; Emoto, Takahiro
• Publication, Distribution, etc.: The International Institute of Anticancer Research
• Publication Date: 2014-09-08
• Publication Date (W3CDTF): 2014-09-08
• Alternative Title: LED光照射による大腸癌細胞制御に関する研究; Effect of LED on Colon Cancer Cells
• Periodical title: Anticancer Research
• No. or year of volume/issue: 34 9
• Volume: 34
• Issue: 9
• Pages: 4709-4716
• ISSN (Periodical Title): ISSN : 0250-7005; ISSN : 1791-7530
• Degree grantor/type: 徳島大学
• Date Granted: 2015-02-26
• Dissertation Number: 甲第2794号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Light-emitting diode; colon cancer; HT29; HCT116 cells
• Target Audience: 一般
• Note (General): Background/Aim: Recent studies have demonstrated the efficacy of irradiation from light emitting diodes (LED) for wound healing, anti-inflammation and anticancer therapies. However, little is known about the effects of visible light in colon cancer cells. The purpose of this study was to evaluate the biological response (including gene expression changes) of human colon cancer cells to different wavelengths of LED irradiation. Materials and Methods: Human colon cancer cells (HT29 or HCT116) were seeded onto laboratory dishes that were then put on LED irradiation equipment with a 465 nm-, 525 nm-, or 635 nm-LED. Irradiation at 15 or 30 mW was performed 10 min/day, each day for 5 days. The cell counting kit8 was then used to measure cell viability. Apoptosis and expression of several mRNAs (caspase, MAPK and autophagy pathway) in HT29 cultures irradiated with 465 nm LED were evaluated via Annexin V/PI and RT-PCR, respectively. Results: Viability of HT29 and HCT116 cells was lower in 465 nm-LED irradiated cultures than in control cultures, but viability of HT29 cells did not differ between control cultures and 525 nm-LED or 635 nm-LED irradiated cultures. Moreover, the expression of FAS, caspase-3, capase-8, and JUK were significantly higher in 465 nm-LED irradiated cultures than in control cultures, and expression of ERK1/2 and LC3 was lower in blue-irradiated cells. Conclusion: LED irradiation at 465 nm inhibited the proliferation of HT29 cells and of HCT116 cells. Notably, LED irradiation at 465 nm promoted apoptosis inHT29 cultures via the extrinsic apoptosis pathway and the MAPK pathway.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/10/109346/20170929143102564556/LID201505141007.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109346/20221213090356681332/k2794_fulltext.pdf fulltext