Propofol-induced relaxation of rat aorta is altered by aging

• Material Type: 博士論文
• Title: Propofol-induced relaxation of rat aorta is altered by aging
• Author/Editor: Sakai, Yoko; Kawahito, Shinji; Takaishi, Kazumi; Mita, Naoji; Kinoshita, Hiroyuki; Hatakeyama, Noboru; Azma, Toshiharu; Nakaya, Yutaka; Kitahata, Hiroshi
• Author Heading: Sakai, Yoko; Kawahito, Shinji; Takaishi, Kazumi; Mita, Naoji; Kinoshita, Hiroyuki; Hatakeyama, Noboru; Azma, Toshiharu; Nakaya, Yutaka; Kitahata, Hiroshi
• Publication Date: 2014-08
• Publication Date (W3CDTF): 2014-08
• Alternative Title: プロポフォールによるラット大動脈の弛緩性は年齢により減弱する; Propofol-induced vasodilation and aging
• Periodical title: The journal of medical investigation : JMI
• No. or year of volume/issue: 61 3-4
• Volume: 61
• Issue: 3-4
• Pages: 278-284
• ISSN (Periodical Title): ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2015-02-26
• Dissertation Number: 甲第2791号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: propofol; vasorelaxation; rat aorta; aging; endothelium
• Target Audience: 一般
• Note (General): Background : Propofol causes vasodilation via endothelium-dependent and -independent mechanisms. Because endothelial function is impaired with aging, the effects of propofol on endothelium-dependent vasodilation might be altered by aging. The aim of this study was thus to determine the effects of aging on vascular responses to propofol. Methods : Young (4-6 weeks old) or adult (16-25 weeks old) rats were anesthetized with sevoflurane. The thoracic aorta was dissected and cut into pieces 3-4mm in length. In some rings, the endothelium was deliberately removed. The ring segment of the aorta was mounted for isometric force recording at a resting tension of 0.5-1.0 g in a 2 ml organ bath, containing Krebs-Ringer bicarbonate buffer. Arteries were precontracted with phenylephrine, and the function of endothelium was confirmed with acetylcholine. Then, we studied the concentration-dependent effects of propofol in endothelium-intact (control group) and -denuded aortic rings (denuded group), as well as those treated with N[ω]-nitro-L-arginine methylester (L-NAME group). Results : Relaxation due to propofol was observed in the control groups of both young and adult rats in a concentration-dependent manner, but the magnitude of relaxation was significantly greater in young rats. In addition, in young rats, relaxation due to propofol was significantly and equally reduced in both L-NAME and denuded groups at all propofol concentrations that we studied (10[-6]-10[-3] M). In adult rats, relaxation due to propofol was quite similar between control and L-NAME groups at all propofol concentrations, whereas it was significantly reduced in the denuded group. Conclusion : These results suggest that endothelium-derived nitric oxide plays an important role in propofol-induced vasodilation in young rats, but not in adult rats.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.61.278
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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