Roles of hypoxia inducible factor-1α in the temporomandibular joint

• Material Type: 博士論文
• Title: Roles of hypoxia inducible factor-1α in the temporomandibular joint
• Author/Editor: Mino-Oka, Akiko; Izawa, Takashi; Shinohara, Takehiro; Mori, Hiroki; Yasue, Akihiro; Tomita, Shuhei; Tanaka, Eiji
• Author Heading: Mino-Oka, Akiko; Izawa, Takashi; Shinohara, Takehiro; Mori, Hiroki; Yasue, Akihiro; Tomita, Shuhei; Tanaka, Eiji
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2016-10-28
• Publication Date (W3CDTF): 2016-10-28
• Alternative Title: 顎関節におけるHIF-1αの役割
• Periodical title: Archives of Oral Biology
• No. or year of volume/issue: 73
• Volume: 73
• Pages: 274-281
• ISSN (Periodical Title): ISSN : 0003-9969
• Degree grantor/type: 徳島大学
• Date Granted: 2017-03-23
• Dissertation Number: 甲第3072号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: TMJ-OA; HIF-1α; Articular cartilage degradation; chondrocyte apoptosis
• Target Audience: 一般
• Note (General): Objective: Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease characterized by permanent cartilage loss. Articular cartilage is maintained in a low-oxygen environment. The chondrocyte response to hypoxic conditions involves expression of hypoxia inducible factor 1α (HIF-1α), which induces chondrocytes to increase expression of vascular endothelial growth factor (VEGF). Here, we investigated the role of HIF-1α in mechanical load effects on condylar cartilage and subchondral bone in heterozygous HIF-1α-deficient mice (HIF-1α+/-). Design: Mechanical stress was applied to the TMJ of C57BL/6NCr wild-type (WT) and HIF-1α+/- mice with a sliding plate for 10 days. Histological analysis was performed by HE staining, Safranin-O/Fast green staining, and immunostaining specific for articular cartilage homeostasis. Results: HIF-1α+/- mice had thinner cartilage and smaller areas of proteoglycan than WT controls, without and with mechanical stress. Mechanical stress resulted in prominent degenerative changes with increased expression of HIF-1α, VEGF, and the apoptosis factor cleaved Caspase-3 in condylar cartilage. Conclusion: Our results indicate that HIF-1a may be important for articular cartilage homeostasis and protective against articular cartilage degradation in the TMJ under mechanical stress condition, therefore HIF-1α could be an important new therapeutic target in TMJ-OA.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.028
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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