大腸上皮細胞に発現するNADPH oxidase1（Nox1）の分子特性

Material type: 文書・図像類

Author: 桑野, 由紀ほか

Publisher: 徳島医学会

Publication date: 2003-04-25

Material Format: Digital

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Note (General)：: NADPH oxidase 1 (Nox1) is an isozyme of gp91-phox predominantly expressed in the human colon. In this study, we have established primary cultures of g...

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Material Type: 文書・図像類
Title: 大腸上皮細胞に発現するNADPH oxidase1（Nox1）の分子特性
Author/Editor: 桑野, 由紀
河原, 司
児玉, 七重
仲井, 沙織
岸, 恭一
六反, 一仁
Author Heading: 桑野, 由紀
河原, 司
児玉, 七重
仲井, 沙織
岸, 恭一
六反, 一仁
Publication, Distribution, etc.: 徳島医学会
Publication Date: 2003-04-25
Publication Date (W3CDTF): 2003-04-25
Alternative Title: Molecular characterization of NADPH oxidase1 expressed on large intestinal epithelial cells
ダイチョウジョウヒサイボウニハツゲンスル NADPH oxidase1 Nox1 ノブンシトクセイ
Periodical title: 四国医学雑誌
No. or year of volume/issue: 59 1-2
Volume: 59
Issue: 1-2
Pages: 45-46
ISSN (Periodical Title): ISSN : 0037-3699
Text Language Code: jpn
Subject Heading: Nox1
large intestinal epithelial cells
Toll-like receptor 5
flagellin
Target Audience: 一般
Note (General): NADPH oxidase 1 (Nox1) is an isozyme of gp91-phox predominantly expressed in the human colon. In this study, we have established primary cultures of guinea pig large intestinal epithelial cells (LIEC). A great majority of the cultured cells (>90%) was surface mucous cells containing periodic acid-Schiff reaction-positive granules. Vimentin-positive fibroblasts were <1%, and macrophages were not contaminated. LIEC spontaneously produced superoxide anion (O2 －) at about 160 nmol/mg protein/h. O2 －-dependent formation of blue formazan particles from nitroblue tetrazolium was observed only on surface of mucous-producing cells, and these cells expressed Nox1 protein at plasma membrane and in the cytoplasm. They expressed p67-phox, p22-phox, and rac1, but not gp91-phox, p47-phox, p40-phox, and rac 2. Immunohistochemistry showed that Nox1, p 67-phox, and p 22-phox were predominantly expressed in surface mucous cells of human and guinea pig colonic mucosa. Human colon cancer cell lines (Caco2, T84, and HT29 cells) expressed Nox1, p22-phox, and rac1, but not the other NADPH components. These cells secreted O2 － at <5 nmol/mg protein/h. Caco2 cells possessed Toll-like receptor 5, and flagellin (FliC) from Salmonella enteritidis phosphorylated transforming growth factor-β-activated kinase 1 (TAK1) and TAK1-binding protein 1, and significantly up-regulated O2 － production. These results suggest that Nox1 expressed in colonic epithelial cells may regulate interactions between pathogenic bacteria and epithelial cells for host defense.
Format (IMT): application/pdf
Source: https://repo.lib.tokushima-u.ac.jp/files/public/11/110583/20170929150909516401/sam_59_1-2_45.pdf
https://repo.lib.tokushima-u.ac.jp/files/public/11/110583/20170929150909516401/sam_59_1-2_45.pdf
Access Restrictions: 限定公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ