Genetic and Phenotypic Changes of Thymus Tissue in Mice during Pregnancy

• Material Type: 博士論文
• Title: Genetic and Phenotypic Changes of Thymus Tissue in Mice during Pregnancy
• Author/Editor: Yamada, Koichi; Arakaki, Rieko; Yamada, Akiko; Kudo, Yasusei; Ishimaru, Naozumi
• Author Heading: Yamada, Koichi; Arakaki, Rieko; Yamada, Akiko; Kudo, Yasusei; Ishimaru, Naozumi
• Publication, Distribution, etc.: 四国歯学会
• Publication Date: 2017-06-30
• Publication Date (W3CDTF): 2017-06-30
• Alternative Title: 妊娠期におけるマウス胸腺組織の遺伝子発現変化の解析
• Periodical title: Journal of Oral Health and Biosciences
• No. or year of volume/issue: 30 1
• Volume: 30
• Issue: 1
• Pages: 26-32
• ISSN (Periodical Title): ISSN : 2189-6682
• Degree grantor/type: 徳島大学
• Date Granted: 2017-03-09
• Dissertation Number: 甲第3077号
• Degree Type: 博士（学術）
• Text Language Code: eng
• Subject Heading: thymus; gene expression; pregnancy; IGFBP5
• Target Audience: 一般
• Note (General): The maternal immune system during pregnancy is largely changed to maintain immune tolerance against the embryo. Although thymus tissues, which generate T cells, are also dynamically altered during pregnancy, the precise phenotype of thymic T cells and gene alteration in thymus tissues remain to be completely elucidated. In this study, we performed a comprehensive analysis of gene expression and assessed T-cell phenotypic changes in the thymus of mice during pregnancy. Thymus tissues of female C57BL/6 mice during pregnancy were resected to investigate T-cell phenotypes using flow cytometric and immunofluorescence analyses and gene expression using a DNA microarray. Decreased weight of thymus tissues was observed during gestation. Cell numbers of total populations of thymic T cells in pregnant mice were significantly decreased compared with those in non-pregnant mice. DNA microarray and RT-PCR analyses revealed several upregulated genes in the thymus tissues of pregnant mice. Among them, the insulin growth factor-binding protein 5 (IGFBP5) was the most upregulated gene, and its increased protein expression of macrophages was confirmed by immunofluorescence analysis. In summary, a unique change in gene expression was observed in transient atrophy of thymus tissues during pregnancy. The change in gene expression, including that in IGFBP5 of macrophages, may influence thymic differentiation of T cells to maintain an immunological tolerance during pregnancy.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.20738/johb.30.1_26
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/111065/20190820174727126255/LID201704261004.pdf fulltext

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