A Novel Method of DAPI Staining for Differential Diagnosis of Renal Amyloidosis

• Material Type: 博士論文
• Title: A Novel Method of DAPI Staining for Differential Diagnosis of Renal Amyloidosis
• Author/Editor: Matsuura, Motokazu; Abe, Hideharu; Tominaga, Tatsuya; Sakurai, Akiko; Murakami, Taichi; Kishi, Seiji; Bando, Yoshimi; Minakuchi, Jun; Nagai, Kojiro; Doi, Toshio
• Author Heading: Matsuura, Motokazu; Abe, Hideharu; Tominaga, Tatsuya; Sakurai, Akiko; Murakami, Taichi; Kishi, Seiji; Bando, Yoshimi; Minakuchi, Jun; Nagai, Kojiro; Doi, Toshio
• Publication, Distribution, etc.: Faculty of Medicine Tokushima University
• Publication Date: 2017-08
• Publication Date (W3CDTF): 2017-08
• Alternative Title: DAPI染色による腎アミロイドーシスの新しい鑑別方法; Diagnosis of renal AL amyloidosis using DAPI
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 64 3-4
• Volume: 64
• Issue: 3-4
• Pages: 217-221
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2017-11-30
• Dissertation Number: 乙第2099号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Amyloidosis; DAPI; Renal amyloidosis; AL amyloidosis; AA amyloidosis
• Target Audience: 一般
• Note (General): Amyloidosis is often overlooked because its clinical manifestations can mimic those of more-common diseases. It is important to get a precise diagnosis as early as possible for the prevention of further organ damages. Amyloidosis is a disorder caused by deposition of insoluble abnormal amyloid. The kidney is a frequent site of amyloid deposition. The amyloid fibrils have a characteristic appearance and generate birefringence under polarized light when stained with the Congo red dye. Classification of amyloidosis is based on the precursor protein that forms the amyloid fibrils and the distribution of amyloid deposits as either systemic or localized. Involvement of amyloid fibrils in kidneys mainly occurs as amyloid light-chain (AL) or amyloid A (AA) amyloidosis. The potassium permanganate method with Congo red staining was once used widely to discriminate AL and AA amyloidoses, but thismethod has a problem of false positive results. We found that extracellular and cytoplasmic glomerular 4’,6-diamidino-2-phenylindole (DAPI)-positive areas were clearly consistent with amyloid deposition in AL amyloidosis. In contrast, the overlapping staining was not seen in AA amyloidosis. Therefore, we propose that DAPI staining readily distinguishes AL renal amyloidosis from AA renal amyloidosis as a simple and reproducible histochemical method.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.64.217
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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