Nonconcordant regulation of mitochondrial respiratory complexes in the kidneys of 5/6 nephrectomized mice

• Material Type: 博士論文
• Title: Nonconcordant regulation of mitochondrial respiratory complexes in the kidneys of 5/6 nephrectomized mice
• Author/Editor: Nakashita, Yukie; Sano, Tomoya; Yoshioka, Hiroaki; Shimada, Tomoki; Hori, Ryotaro; Usami, Makoto; Hamada, Yasuhiro
• Author Heading: Nakashita, Yukie; Sano, Tomoya; Yoshioka, Hiroaki; Shimada, Tomoki; Hori, Ryotaro; Usami, Makoto; Hamada, Yasuhiro
• Publication, Distribution, etc.: Faculty of Medicine Tokushima University
• Publication Date: 2017-08
• Publication Date (W3CDTF): 2017-08
• Alternative Title: 5/6腎摘マウスの腎臓におけるミトコンドリア呼吸鎖複合体の不均衡; Renal mitochondrial complex in 5/6 Nx mice
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 64 3-4
• Volume: 64
• Issue: 3-4
• Pages: 255-261
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2018-02-22
• Dissertation Number: 甲第3137号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: db/m-5/6 nephrectomized mice; db/db mice; glomerular hyperfiltration; mitochondrial respiratory complex
• Target Audience: 一般
• Note (General): Hyperglycemia induces nonconcordant regulation of renal mitochondrial respiratory complexes, increases oxidative stress, and causes diabetic nephropathy. Hypertension is a complication associated with diabetes and involves glomerular hyperfiltration, the effects of which on mitochondrial respiratory complexes are not well understood. To investigate the effect of glomerular hyperfiltration on renal mitochondrial respiratory complexes, we used the 5/6 nephrectomized BKS.Cg-Dock7m+/+Leprdb/J, Dock7m+/+Leprdb mice (db/m-5/6Nx mice) as a model for glomerular hyperfiltration. The BKS.Cg-Dock7m+/+Leprdb/J, +Leprdb/+Leprdb mice (db/db mice), a model for type 2 diabetes, was used as the positive control. We investigated the activities and protein levels of the mitochondrial complex, and themitochondrial DNA and adenosine triphosphate content in the kidneys of these models. Blood chemistry and renal histopathological examination were performed for characterization of the disease. Both models showed expansion of the mesangial matrix of the glomeruli, which is indicative of glomerular hyperfiltration. The activities of complexes I and IV and the protein levels of complexes I and III were nonconcordant in db/m-5/6Nx mice. In conclusion, we demonstrated that nonconcordant regulation of mitochondrial complexes in db/m-5/6Nx mice involved with glomerular hyperfiltration. The progression and/or severity of nephropathymight be affected through a synergistic effect of mitochondrial dysfunction in hyperglycemia and glomerular hyperfiltration.
• Format (IMT): application/pdf
• Source: fulltext; http://repo.lib.tokushima-u.ac.jp/files/public/11/111127/20190726140324901904/k3137_abstract_review.pdf; http://repo.lib.tokushima-u.ac.jp/files/public/11/111127/20190730172351957204/k3137_fulltext.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.64.255
• Periodical Title (NCID): AA11166929; https://ci.nii.ac.jp/ncid/AA11166929; AA12022913; https://ci.nii.ac.jp/ncid/AA12022913
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ