Effect of Npt2b deletion on intestinal and renal inorganic phosphate (Pi) handling

• Material Type: 博士論文
• Title: Effect of Npt2b deletion on intestinal and renal inorganic phosphate (Pi) handling
• Author/Editor: Ikuta, Kayo; Segawa, Hiroko; Sasaki, Shohei; Hanazaki, Ai; Fujii, Toru; Kushi, Aoi; Kawabata, Yuka; Kirino, Ruri; Sasaki, Sumire; Noguchi, Miwa; Kaneko, Ichiro; Tatsumi, Sawako; Ueda, Otoya; Wada, Naoko A.; Tateishi, Hiromi; Kakefuda, Mami; Kawase, Yosuke; Ohtomo, Shuichi; Ichida, Yasuhiro; Maeda, Akira; Jishage, Kou-ichi; Horiba, Naoshi; Miyamoto, Ken-ichi
• Author Heading: Ikuta, Kayo; Segawa, Hiroko; Sasaki, Shohei; Hanazaki, Ai; Fujii, Toru; Kushi, Aoi; Kawabata, Yuka; Kirino, Ruri; Sasaki, Sumire; Noguchi, Miwa; Kaneko, Ichiro; Tatsumi, Sawako; Ueda, Otoya; Wada, Naoko A.; Tateishi, Hiromi; Kakefuda, Mami; Kawase, Yosuke; Ohtomo, Shuichi; Ichida, Yasuhiro; Maeda, Akira; Jishage, Kou-ichi; Horiba, Naoshi; Miyamoto, Ken-ichi
• Publication, Distribution, etc.: Japanese Society of Nephrology|Springer
• Publication Date: 2017-11-11
• Publication Date (W3CDTF): 2017-11-11
• Alternative Title: 腸管および腎臓でのリン代謝におけるNpt2b欠損の影響; Conditional deletion of Npt2b in phosphate transport
• Periodical title: Clinical and Experimental Nephrology
• No. or year of volume/issue: 22 3
• Volume: 22
• Issue: 3
• Pages: 517-528
• ISSN (Periodical Title): ISSN : 1342-1751; ISSN : 1437-7799
• Degree grantor/type: 徳島大学
• Date Granted: 2018-03-23
• Dissertation Number: 甲第3139号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Intestine; Transcellular transport-paracellular transport
• Target Audience: 一般
• Note (General): Background Hyperphosphatemia is common in chronic kidney disease and is associated with morbidity and mortality. The intestinal Na+-dependent phosphate transporter Npt2b is thought to be an important molecular target for the prevention of hyperphosphatemia. The role of Npt2b in the net absorption of inorganic phosphate (Pi), however, is controversial. Methods In the present study, we made tamoxifen-inducible Npt2b conditional knockout (CKO) mice to analyze systemic Pi metabolism, including intestinal Pi absorption. Results Although the Na+-dependent Pi transport in brush-border membrane vesicle uptake levels were significantly decreased in the distal intestine of Npt2b CKO mice compared with control mice, plasma Pi and fecal Pi excretion levels were not significantly different. Data obtained using the intestinal loop technique showed that Pi uptake in Npt2b CKO mice was not affected at a Pi concentration of 4 mM, which is considered the typical luminal Pi concentration after meals in mice. Claudin, which may be involved in paracellular pathways, as well as claudin-2, 12, and 15 protein levels were significantly decreased in the Npt2b CKO mice. Thus, Npt2b deficiency did not affect Pi absorption within the range of Pi concentrations that normally occurs after meals. Conclusion These findings indicate that abnormal Pi metabolism may also be involved in tight junction molecules such as Cldns that are affected by Npt2b deficiency.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © Japanese Society of Nephrology 2017 This is a post-peer-review, pre-copyedit version of an article published in Clinical and Experimental Nephrology. The final publication is available at Springer via https://doi.org/10.1007/s10157-017-1497-3
• Related Material (DOI): 10.1007/s10157-017-1497-3
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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