Detecting Enteral Nutrition Residues and Microorganism Proliferation in Feeding Tubes via Real‐Time Imaging

• Material Type: 博士論文
• Title: Detecting Enteral Nutrition Residues and Microorganism Proliferation in Feeding Tubes via Real‐Time Imaging
• Author/Editor: Yamaoka, Ippei; Kagawa, Tomohiro; Mizugai, Kazuya; Ebisu, Goro
• Author Heading: Yamaoka, Ippei; Kagawa, Tomohiro; Mizugai, Kazuya; Ebisu, Goro
• Publication, Distribution, etc.: Wiley|American Society for Parenteral and Enteral Nutrition
• Publication Date: 2016-11-04
• Publication Date (W3CDTF): 2016-11-04
• Alternative Title: リアルタイムイメージングによる栄養チューブ内における流動食残渣と微生物増殖の検出
• Periodical title: Nutrtion in Clinical Practice
• No. or year of volume/issue: 32 2
• Volume: 32
• Issue: 2
• Pages: 282-287
• ISSN (Periodical Title): ISSN : 1941-2452
• Degree grantor/type: 徳島大学
• Date Granted: 2018-09-27
• Dissertation Number: 乙第2104号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: bacteria; biofilms; bioluminescence; enteral nutrition; feeding tubes; fluorescence
• Target Audience: 一般
• Note (General): Background: Enteral nutrition (EN) residues that persist in feeding tubes provide substrates for microorganisms to proliferate and occlude the tubes. Visible EN residues in tubes are easily identified, but smaller residues can persist. We developed a new imaging technique to visualize EN residues and proliferation of microorganisms in feeding tubes. Materials and Methods: Feeding tubes containing EN labelled with fluorescent dye and EN with or without various types or amounts of thickeners were flushed once with water and then the tubes were seeded with Pseudomonas aeruginosa Xen5 with recombinant luciferase DNA. Because EN fluoresces intrinsically, EN in the feeding tubes without fluorescent dye was repeatedly flushed until the intrinsic fluorescence levels reached background levels. Fluorescent images of EN residues and bioluminescent images of microorganisms were acquired using an optical imaging system. Results: Fluorescence images showed that the amount of EN residues increased at various sites in tubes depending on EN viscosity and the thickening agent, and bioluminescence images showed that microorganism proliferation was associated with a commensurate increase in EN residues. The intrinsic fluorescence of EN also enabled the detection of EN residues in tubes even in the absence of fluorescence dye. Higher EN viscosity required more flushes to reach undetectable levels. Conclusion: EN residues and microorganism proliferation in enteral feeding tubes were detected on fluorescence and bioluminescence images, respectively. This simplified approach allowed the real-time visualization of EN residues and microorganisms in feeding tubes.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1177/0884533616675189
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/112841/20181212134742323214/o2104_abstract_review.pdf fulltext

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