A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis

• Material Type: 博士論文
• Title: A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis
• Author/Editor: Sasaki, Shohei; Segawa, Hiroko; Hanazaki, Ai; Kirino, Ruri; Fujii, Toru; Ikuta, Kayo; Noguchi, Miwa; Sasaki, Sumire; Koike, Megumi; Tanifuji, Kazuya; Shiozaki, Yuji; Kaneko, Ichiro; Tatsumi, Sawako; Shimohata, Takaaki; Kawai, Yoshichika; Narisawa, Sonoko; Millán, José Luis; Miyamoto, Ken-ichi
• Author Heading: Sasaki, Shohei; Segawa, Hiroko; Hanazaki, Ai; Kirino, Ruri; Fujii, Toru; Ikuta, Kayo; Noguchi, Miwa; Sasaki, Sumire; Koike, Megumi; Tanifuji, Kazuya; Shiozaki, Yuji; Kaneko, Ichiro; Tatsumi, Sawako; Shimohata, Takaaki; Kawai, Yoshichika; Narisawa, Sonoko; Millán, José Luis; Miyamoto, Ken-ichi
• Publication, Distribution, etc.: S. Karger AG
• Publication Date: 2018-09-10
• Publication Date (W3CDTF): 2018-09-10
• Alternative Title: 小腸のリン恒常性における小腸型アルカリホスファターゼ(Akp3)の役割について
• Periodical title: Kidney and Blood Pressure Research
• No. or year of volume/issue: 43 5
• Volume: 43
• Issue: 5
• Pages: 1409-1424
• ISSN (Periodical Title): ISSN : 1420-4096; ISSN : 1423-0143
• Degree grantor/type: 徳島大学
• Date Granted: 2018-09-27
• Dissertation Number: 甲第3220号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Hyperphosphatemia; CKD-MBD; Phosphate transporter
• Target Audience: 一般
• Note (General): Background/Aims: Hyperphosphatemia is a serious complication of late-stage chronic kidney disease (CKD). Intestinal inorganic phosphate (Pi) handling plays an important role in Pi homeostasis in CKD. We investigated whether intestinal alkaline phosphatase 3 (Akp3), the enzyme that hydrolyzes dietary Pi compounds, is a target for the treatment of hyperphosphatemia in CKD. Methods: We investigated Pi homeostasis in Akp3 knockout mice (Akp3-/-). We also studied the progression of renal failure in an Akp3-/- mouse adenine treated renal failure model. Plasma, fecal, and urinary Pi and Ca concentration were measured with commercially available kit, and plasma fibroblast growth factor 23, parathyroid hormone, and 1,25(OH)2D3 concentration were measured with ELISA. Brush border membrane vesicles were prepared from mouse intestine using the Ca2+ precipitation method and used for Pi transport activity and alkaline phosphatase activity. In vivo intestinal Pi absorption was measured with oral 32P administration. Results: Akp3-/- mice exhibited reduced intestinal type II sodium-dependent Pi transporter (Npt2b) protein levels and Na-dependent Pi co-transport activity. In addition, plasma active vitamin D levels were significantly increased in Akp3-/- mice compared with wild-type animals. In the adenine-induced renal failure model, Akp3 gene deletion suppressed hyperphosphatemia. Conclusion: The present findings indicate that intestinal Akp3 deletion affects Na+-dependent Pi transport in the small intestine. In the adenine-induced renal failure model, Akp3 is predicted to be a factor contributing to suppression of the plasma Pi concentration.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.
• Related Material (DOI): 10.1159/000493379
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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