Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats

• Material Type: 博士論文
• Title: Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats
• Author/Editor: Fujii, Tatsuya; Kitamura, Yoshiaki; Mizuguchi, Hiroyuki; Okamoto, Kentaro; Sanada, Nanae; Yamada, Takuya; Sugiyama, Manabu; Michinaga, Shotaro; Kitayama, Mika; Fukui, Hiroyuki; Takeda, Noriaki
• Author Heading: Fujii, Tatsuya; Kitamura, Yoshiaki; Mizuguchi, Hiroyuki; Okamoto, Kentaro; Sanada, Nanae; Yamada, Takuya; Sugiyama, Manabu; Michinaga, Shotaro; Kitayama, Mika; Fukui, Hiroyuki; Takeda, Noriaki
• Publication, Distribution, etc.: Elsevier|Japanese Pharmacological Society
• Publication Date: 2018-09-10
• Publication Date (W3CDTF): 2018-09-10
• Alternative Title: ナローバンドUVBがHeLa細胞とアレルギー性鼻炎モデルラットの鼻粘膜におけるヒスタミンH1受容体遺伝子発現の亢進とアポトーシスの誘導に与える影響
• Periodical title: Journal of Pharmacological Sciences
• No. or year of volume/issue: 138 1
• Volume: 138
• Issue: 1
• Pages: 54-62
• ISSN (Periodical Title): ISSN : 1347-8613
• Degree grantor/type: 徳島大学
• Date Granted: 2018-11-22
• Dissertation Number: 甲第3246号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Allergic rhinitis; apoptosis; histamine H1 receptor gene; narrowband-ultraviolet B; phototherapy
• Target Audience: 一般
• Note (General): Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12- myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and upregulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
• Related Material (DOI): 10.1016/j.jphs.2018.08.011
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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