Deciphering defective amelogenesis using in vitro culture systems

• Material Type: 博士論文
• Title: Deciphering defective amelogenesis using in vitro culture systems
• Author/Editor: Arinawati, Dian Yosi; Miyoshi, Keiko; Tanimura, Ayako; Horiguchi, Taigo; Hagita, Hiroko; Noma, Takafumi
• Author Heading: Arinawati, Dian Yosi; Miyoshi, Keiko; Tanimura, Ayako; Horiguchi, Taigo; Hagita, Hiroko; Noma, Takafumi
• Publication, Distribution, etc.: The Society for Biotechnology, Japan|Elsevier
• Publication Date: 2018-02-01
• Publication Date (W3CDTF): 2018-02-01
• Alternative Title: in vitro 細胞培養系によるアメロジェネシス不全メカニズムの解読; IN VITRO MODELING OF AMELOGENESIS
• Periodical title: Journal of Bioscience and Bioengineering
• No. or year of volume/issue: 125 4
• Volume: 125
• Issue: 4
• Pages: 479-489
• ISSN (Periodical Title): ISSN : 1389-1723
• Degree grantor/type: 徳島大学
• Date Granted: 2018-09-13
• Dissertation Number: 甲第3205号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Amelogenesis imperfecta; Cell-cell interaction; Cell-matrix interaction; Dental epithelial cell; Genetic disease; In vitro amelogenesis imperfecta model; Phenotypic screening; Sp6
• Target Audience: 一般
• Note (General): The conventional two-dimensional (2D) in vitro culture system is frequently used to analyze the gene expression with or without extracellular signals. However, the cells derived from primary culture and cell lines frequently deviate the gene expression profile compared to the corresponding in vivo samples, which sometimes misleads the actual gene regulation in vivo. To overcome this gap, we developed the comparative 2D and 3D in vitro culture systems and applied them to the genetic study of amelogenesis imperfecta (AI) as a model. Recently, we found specificity protein 6 (Sp6) mutation in an autosomal-recessive AI rat that was previously named AMI. We constructed 3D structure of ARE-B30 cells (AMI-derived rat dental epithelial cells) or G5 (control wild type cells) combined with RPC-C2A cells (rat pulp cell line) separated by the collagen membrane, while in 2D structure, ARE-B30 or G5 was cultured with or without the collagen membrane. Comparative analysis of amelogenesis-related gene expression in ARE-B30 and G5 using our 2D and 3D in vitro systems revealed distinct expression profiles, showing the causative outcomes. Bone morphogenetic protein 2 and follistatin were reciprocally expressed in G5, but not in ARE-B30 cells. All-or-none expression of amelotin, kallikrein-related peptidase 4, and nerve growth factor receptor was observed in both cell types. In conclusion, our in vitro culture systems detected the phenotypical differences in the expression of the stage-specific amelogenesis-related genes. Parallel analysis with 2D and 3D culture systems may provide a platform to understand the molecular basis for defective amelogenesis caused by Sp6 mutation.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): ©2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.( http://creativecommons.org/licenses/by-nc-nd/4.0 )
• Related Material (DOI): 10.1016/j.jbiosc.2017.11.009
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

Table of Contents

https://repo.lib.tokushima-u.ac.jp/files/public/11/112952/20190828121029187559/k3205_abstract.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/112952/20190828121029848659/k3205_review.pdf fulltext

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