Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway

• Material Type: 博士論文
• Title: Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway
• Author/Editor: Mineda, Ayuka; Nishimura, Masato; Kagawa, Tomohiro; Takiguchi, Eri; Kawakita, Takako; Abe, Akiko; Irahara, Minoru
• Author Heading: Mineda, Ayuka; Nishimura, Masato; Kagawa, Tomohiro; Takiguchi, Eri; Kawakita, Takako; Abe, Akiko; Irahara, Minoru
• Publication, Distribution, etc.: Spandidos Publications
• Publication Date: 2019-01-28
• Publication Date (W3CDTF): 2019-01-28
• Alternative Title: レスベラトロールは子宮肉腫細胞において、wntシグナルを抑制することによりアポトーシスを誘導し、細胞増殖を抑制する
• Periodical title: Experimental and Therapeutic Medicine
• No. or year of volume/issue: 17 3
• Volume: 17
• Issue: 3
• Pages: 2242-2246
• ISSN (Periodical Title): ISSN : 1792-0981; ISSN : 1792-1015
• Degree grantor/type: 徳島大学
• Date Granted: 2019-03-22
• Dissertation Number: 甲第3254号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: resveratrol; uterine sarcoma; Wnt signaling pathway; β-catenin; glycogen synthase kinase-3β
• Target Audience: 一般
• Note (General): Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © Mineda et al. This is an open access article distributed under the terms of Creative Commons Attribution License.( https://creativecommons.org/licenses/by-nc-nd/4.0/ )
• Related Material (DOI): 10.3892/etm.2019.7209
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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