Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney

• Material Type: 博士論文
• Title: Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney
• Author/Editor: Fujii, Toru; Shiozaki, Yuji; Segawa, Hiroko; Nishiguchi, Shiori; Hanazaki, Ai; Noguchi, Miwa; Kirino, Ruri; Sasaki, Sumire; Tanifuji, Kazuya; Koike, Megumi; Yokoyama, Mizuki; Arima, Yuki; Kaneko, Ichiro; Tatsumi, Sawako; Ito, Mikiko; Miyamoto, Ken-ichi
• Author Heading: Fujii, Toru; Shiozaki, Yuji; Segawa, Hiroko; Nishiguchi, Shiori; Hanazaki, Ai; Noguchi, Miwa; Kirino, Ruri; Sasaki, Sumire; Tanifuji, Kazuya; Koike, Megumi; Yokoyama, Mizuki; Arima, Yuki; Kaneko, Ichiro; Tatsumi, Sawako; Ito, Mikiko; Miyamoto, Ken-ichi
• Publication, Distribution, etc.: Japanese Society of Nephrology|Springer
• Publication Date: 2018-10-13
• Publication Date (W3CDTF): 2018-10-13
• Alternative Title: ヒト腎臓におけるリン代謝機構を理解するためのオポッサム腎NaPi-IIc (Na+依存性Piトランスポーター)の解析
• Periodical title: Clinical and Experimental Nephrology
• No. or year of volume/issue: 23 3
• Volume: 23
• Issue: 3
• Pages: 313-324
• ISSN (Periodical Title): ISSN : 1342-1751; ISSN : 1437-7799
• Degree grantor/type: 徳島大学
• Date Granted: 2019-03-22
• Dissertation Number: 甲第3273号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Phosphate; Transporter; Proximal tubule; Hereditary hypophosphatemic rickets with hypercalciuria
• Target Audience: 一般
• Note (General): Background The role of Na+- dependent inorganic phosphate (Pi) transporters in the human kidney is not fully clarified. Hereditary hypophosphatemic rickets with hypercalciuria (HHRH) is caused by loss-of-function mutations in the IIc Na+- dependent Pi transporter (NPT2c/Npt2c/NaPi-IIc) gene. Another Na+- dependent type II transporter, (NPT2A/Npt2a/NaPi-IIa), is also important for renal Pi reabsorption in humans. In mice, Npt2c deletion does not lead to hypophosphatemia and rickets because Npt2a compensates for the impaired Pi reabsorption. To clarify the differences between mouse and human, we investigated the relation between NaPi-IIa and NaPi-IIc functions in opossum kidney (OK) cells. Methods We cloned NaPi-IIc from OK cells and created opossum NaPi-IIc (oNaPi-IIc) antibodies. We used oNaPi-IIc small interference (si)RNA and investigated the role of NaPi-IIc in Pi transport in OK cells. Results We cloned opossum kidney NaPi-IIc cDNAs encoding 622 amino acid proteins (variant1) and examined their pH- and sodium-dependency. The antibodies reacted specifically with 75-kDa and 150-kDa protein bands, and the siRNA of NaPi-IIc markedly suppressed endogenous oNaPi-IIc in OK cells. Treatment with siRNA significantly suppressed the expression of NaPi-4 (NaPi-IIa) protein and mRNA. oNaPi-IIc siRNA also suppressed Na+/H+ exchanger regulatory factor 1 expression in OK cells. Conclusion These findings suggest that NaPi-IIc is important for the expression of NaPi-IIa (NaPi-4) protein in OK cells. Suppression of Npt2c may downregulate Npt2a function in HHRH patients.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © Japanese Society of Nephrology 2018
• Related Material (DOI): 10.1007/s10157-018-1653-4
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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