Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet

• Material Type: 博士論文
• Title: Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet
• Author/Editor: Raju, Resmi; Oshima, Masamitsu; Inoue, Miho; Morita, Tsuyoshi; Huijiao, Yan; Waskitho, Arief; Baba, Otto; Inoue, Masahisa; Matsuka, Yoshizo
• Author Heading: Raju, Resmi; Oshima, Masamitsu; Inoue, Miho; Morita, Tsuyoshi; Huijiao, Yan; Waskitho, Arief; Baba, Otto; Inoue, Masahisa; Matsuka, Yoshizo
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2020-02-03
• Publication Date (W3CDTF): 2020-02-03
• Alternative Title: 骨－歯根膜線維の複合組織形成による三次元的な歯周組織再生技術の開発
• Periodical title: Scientific Reports
• No. or year of volume/issue: 10
• Volume: 10
• Pages: 1656-
• ISSN (Periodical Title): ISSN : 2045-2322
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-23
• Dissertation Number: 甲第3381号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Cell sheet; Periodontal tissue; Regeneration; Periodontal ligament cell
• Target Audience: 一般
• Note (General): Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• Related Material (DOI): 10.1038/s41598-020-58222-0
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

Table of Contents

https://repo.lib.tokushima-u.ac.jp/files/public/11/114213/20200212112613804827/srep_10_1656.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/114213/20200416153452454356/k3381_abstract.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/114213/2020041615345312608/k3381_review.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/114213/20200416153453368978/k3381_fulltext.pdf fulltext