Association of increased renal Cyp24a1 gene expression with low plasma 1,25-dihydroxyvitamin D levels in rats with streptozotocin-induced diabetes

• Material Type: 博士論文
• Title: Association of increased renal Cyp24a1 gene expression with low plasma 1,25-dihydroxyvitamin D levels in rats with streptozotocin-induced diabetes
• Author/Editor: Tajiri, Mari; Nakahashi, Otoki; Kagawa, Tomohiro; Masuda, Masashi; Ohminami, Hirokazu; Iwano, Masayuki; Takeda, Eiji; Taketani, Yutaka; Yamamoto, Hironori
• Author Heading: Tajiri, Mari; Nakahashi, Otoki; Kagawa, Tomohiro; Masuda, Masashi; Ohminami, Hirokazu; Iwano, Masayuki; Takeda, Eiji; Taketani, Yutaka; Yamamoto, Hironori
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2020-01-01
• Publication Date (W3CDTF): 2020-01-01
• Alternative Title: ストレプトゾトシン誘導性糖尿病ラットにおける腎Cyp24a1遺伝子発現上昇と血漿1,25-ジヒドロキシビタミンD濃度低下との関連
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• No. or year of volume/issue: 66 1
• Volume: 66
• Issue: 1
• Pages: 49-56
• ISSN (Periodical Title): ISSN : 0912-0009; ISSN : 1880-5086
• Degree grantor/type: 徳島大学
• Date Granted: 2019-11-28
• Dissertation Number: 甲第3340号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: vitamin D; CYP24A1; CYP24A1-SV; diabetes; streptozotocin
• Target Audience: 一般
• Note (General): Decreases in plasma vitamin D concentrations have been reported in diabetes, although the mechanism involved in this decrease is unclear. Here, we investigated the association between Cyp24a1, a vitamin D catabolic enzyme, and abnormalities in vitamin D metabolism in streptozotocin-induced diabetes rats, an animal model of type 1 diabetes. Plasma 1,25-dihydroxyvitamin D [1,25(OH)2D] levels were significantly lower in streptozotocin-induced diabetes rats and renal Cyp24a1 mRNA expression levels were increased. Western blotting analysis of streptozotocin-induced diabetes rats kidney tissues with anti-CYP24A1 antibody showed a strong signal around 40 kDa, which differs from the predicted 50–55 kDa molecular weight for full-length Cyp24a1 and could represent the Cyp24a1-splicing variant that lacks exons 1 and 2. We observed high levels of renal Cyp24a1-splicing variant mRNA expression in streptozotocin-induced diabetes rats. We also confirmed transcriptional up-regulation of endogenous Cyp24a1 mRNA expression through glucocorticoid receptors by glucocorticoid in opossum kidney proximal cells. Taken together, our results indicated that high Cyp24a1 expression levels may play a role in the decrease of plasma 1,25(OH)2D levels in streptozotocin-induced diabetes rats. High plasma corticosterone levels in diabetes may affect transcriptional regulation to promote increases in Cyp24a1 expression.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2020 Society for Free Radical Research Japan
• Related Material (DOI): 10.3164/jcbn.19-79
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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