S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-like Cells

• Material Type: 博士論文
• Title: S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-like Cells
• Author/Editor: Takagi, Ryosuke; Sakamoto, Eijiro; Kido, Jun-ichi; Inagaki, Yuji; Hiroshima, Yuka; Naruishi, Koji; Yumoto, Hiromichi
• Author Heading: Takagi, Ryosuke; Sakamoto, Eijiro; Kido, Jun-ichi; Inagaki, Yuji; Hiroshima, Yuka; Naruishi, Koji; Yumoto, Hiromichi
• Publication, Distribution, etc.: Hindawi
• Publication Date: 2020-02-20
• Publication Date (W3CDTF): 2020-02-20
• Alternative Title: S100A9は、骨細胞様細胞においてMAPKsおよびSTAT3シグナル伝達経路を介してIL-6およびRANKLの発現を増加させる
• Periodical title: BioMed Research International
• No. or year of volume/issue: 2020
• Volume: 2020
• Pages: 7149408-
• ISSN (Periodical Title): ISSN : 2314-6133; ISSN : 2314-6141
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-23
• Dissertation Number: 甲第3380号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Osteocyte; Calprotectin; S100A9; IL-6; RANKL
• Target Audience: 一般
• Note (General): Objective: Calprotectin is hetero-complex of S100A8 and S100A9 and mainly secreted from neutrophils, monocytes and chondrocytes in inflammatory condition. Calprotectin binds to RAGE and TLR4, and induces the expression of pro-inflammatory chemokines and cytokines in various cells. Periodontitis is chronic inflammatory disease to lead gingival inflammation and alveolar bone resorption. Calprotectin levels in gingival crevicular fluid of periodontitis patients are higher than healthy patients. In the present study, the effects of S100A8 and S100A9 on the expressions of pro-inflammatory cytokines and bone metabolism related factor in mouse osteocyte like cells (MLO-Y4-A2) were investigated. Design: MLO-Y4-A2 cells were treated with S100A8 and S100A9, and the expressions of RAGE, TLR4, RANKL and several inflammatory cytokines were analyzed by PCR and Western blotting or ELISA methods. To investigate the intracellular signaling pathways, phosphorylation of MAPK and STAT3 was determined by Western blotting, and chemical specific inhibitors and siRNAs were used. Results: Expressions of IL-6 and RANKL were increased by treatment with S100A9 but not S100A8. However, both S100A8 and S100A9 did not changed expression of IL-1β, IL-8 and TNF-α. Although RAGE and TLR4 expressions were not up-regulated by S100A9 treatment, transfection of siRNA for RAGE and TLR4 significantly decreased IL-6 and RANKL expressions. In addition, S100A9 activated p38, ERK and STAT3 signaling pathways, and inhibitors for these factors significantly decreased S100A9 induced IL-6 and RANKL expressions. Conclusions: These results indicated that S100A9 induces IL-6 and RANKL production via engagement with RAGE and TLR4 signalings in osteocytes and suggested that S100A9 may play important roles in the periodontal alveolar bone destruction.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2020 Ryosuke Takagi et al. This is an open access article distributed under the Creative Commons Attribution License,( https://creativecommons.org/licenses/by/4.0/ ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
• Related Material (DOI): 10.1155/2020/7149408
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

Table of Contents

https://repo.lib.tokushima-u.ac.jp/files/public/11/114641/20200420161154121321/bmri_2020_7149408.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/114641/20200427151400630021/k3380_abstract.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/114641/20200427151401180714/k3380_review.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/114641/20200427151401496129/k3380_fulltext.pdf fulltext