Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1

• Material Type: 博士論文
• Title: Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1
• Author/Editor: Miyazaki, Aya; Sugimoto, Asuna; Yoshizaki, Keigo; Kawarabayashi, Keita; Iwata, Kokoro; Kurogoushi, Rika; Kitamura, Takamasa; Otsuka, Kunihiro; Hasegawa, Tomokazu; Akazawa, Yuki; Fukumoto, Satoshi; Ishimaru, Naozumi; Iwamoto, Tsutomu
• Author Heading: Miyazaki, Aya; Sugimoto, Asuna; Yoshizaki, Keigo; Kawarabayashi, Keita; Iwata, Kokoro; Kurogoushi, Rika; Kitamura, Takamasa; Otsuka, Kunihiro; Hasegawa, Tomokazu; Akazawa, Yuki; Fukumoto, Satoshi; Ishimaru, Naozumi; Iwamoto, Tsutomu
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2019-10-14
• Publication Date (W3CDTF): 2019-10-14
• Alternative Title: ピエゾ型機械受容チャネル1による歯の発生過程におけるWNTシグナルと一次繊毛発現の調整
• Periodical title: Scientific Reports
• No. or year of volume/issue: 9
• Volume: 9
• Pages: 14762-
• ISSN (Periodical Title): ISSN : 2045-2322
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-23
• Dissertation Number: 甲第3373号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: PIEZO1; WNT; hydrostatic pressure; odontblast; RUNX2
• Target Audience: 一般
• Note (General): Signal transmission from the mechanical forces to the various intracellular activities is a fundamental process during tissue development. Despite their critical role, the mechanism of mechanical forces in the biological process is poorly understood. In this study, we demonstrated that in the response to hydrostatic pressure (HP), the piezo type mechanosensitive ion channel component 1 (PIEZO1) is a primary mechanosensing receptor for odontoblast differentiation through coordination of the WNT expression and ciliogenesis. In stem cells from human exfoliated deciduous teeth (SHED), HP significantly promoted calcium deposition as well as the expression of odontogenic marker genes, PANX3 and DSPP, and WNT related-genes including WNT5b and WNT16, whereas HP inhibited cell proliferation and enhanced primary cilia expression. WNT signaling inhibitor XAV939 and primary cilia inhibitor chloral hydrate blocked the HP-induced calcium deposition. The PIEZO1 activator Yoda1 inhibited cell proliferation but induced ciliogenesis and WNT16 expression. Interestingly, HP and Yoda1 promoted nuclear translocation of RUNX2, whereas siRNA-mediated silencing of PIEZO1 decreased HP-induced nuclear translocation of RUNX2. Taken together, these results suggest that PIEZO1 functions as a mechanotransducer that connects HP signal to the intracellular signalings during odontoblast differentiation.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2019
• Related Material (DOI): 10.1038/s41598-019-51381-9
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

Table of Contents

http://repo.lib.tokushima-u.ac.jp/files/public/11/114667/20200423145617403498/k3373_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/114667/20200423145617953934/k3373_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/114667/20200423145618274967/k3373_fulltext.pdf fulltext