6-Shogaol Inhibits Advanced Glycation End-Products-Induced IL-6 and ICAM-1 Expression by Regulation Oxidative Responses in Human Gingival Fibroblasts

• Material Type: 博士論文
• Title: 6-Shogaol Inhibits Advanced Glycation End-Products-Induced IL-6 and ICAM-1 Expression by Regulation Oxidative Responses in Human Gingival Fibroblasts
• Author/Editor: Nonaka, Kohei; Bando, Mika; Sakamoto, Eijiro; Inagaki, Yuji; Naruishi, Koji; Yumoto, Hiromichi; Kido, Jun-ichi
• Author Heading: Nonaka, Kohei; Bando, Mika; Sakamoto, Eijiro; Inagaki, Yuji; Naruishi, Koji; Yumoto, Hiromichi; Kido, Jun-ichi
• Publication, Distribution, etc.: MDPI
• Publication Date: 2019-10-15
• Publication Date (W3CDTF): 2019-10-15
• Alternative Title: ショウガオールはヒト歯肉線維芽細胞において酸化ストレス反応の調節を介してAGEs誘導性のIL-6およびICAM-1産生を抑制する
• Periodical title: Molecules
• No. or year of volume/issue: 24 20
• Volume: 24
• Issue: 20
• Pages: 3705-
• ISSN (Periodical Title): ISSN : 1420-3049
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-23
• Dissertation Number: 甲第3377号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: advanced glycation end-products; 6-shogaol; ROS; antioxidant enzyme; IL-6; ICAM-1; human gingival fibroblasts
• Target Audience: 一般
• Note (General): Advanced glycation end-products (AGEs) cause diabetes mellitus (DM) complications and accumulate more highly in periodontal tissues of patients with periodontitis and DM. AGEs aggravate periodontitis with DM by increasing the expression of inflammation-related factors in periodontal tissues. 6-Shogaol, a major compound in ginger, has anti-inflammatory and anti-oxidative activities. However, the influence of shogaol on DM-associated periodontitis is not well known. In this study, the effects of 6-shogaol on AGEs-induced oxidative and anti-oxidative responses, and IL-6 and ICAM-1 expression in human gingival fibroblasts (HGFs) were investigated. When HGFs were cultured with 6-shogaol and AGEs, the activities of reactive oxygen species (ROS) and antioxidant enzymes (heme oxygenase-1 [HO-1] and NAD(P)H quinone dehydrogenase 1 [NQO1]), and IL-6 and ICAM-1 expressions were investigated. RAGE expression and phosphorylation of MAPKs and NF-κB were examined by western blotting. 6-Shogaol significantly inhibited AGEs-induced ROS activity, and increased HO-1 and NQO1 levels compared with the AGEs-treated cells. The AGEs-stimulated expression levels of receptor of AGE (RAGE), IL-6 and ICAM-1 and the phosphorylation of p38, ERK and p65 were attenuated by 6-shogaol. These results suggested that 6-shogaol inhibits AGEs-induced inflammatory responses by regulating oxidative and anti-oxidative activities and may have protective effects on periodontitis with DM.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• Related Material (DOI): 10.3390/molecules24203705
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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