Factors secreted from dental pulp stem cells show multifaceted benefits for treating experimental temporomandibular joint osteoarthritis

• Material Type: 博士論文
• Title: Factors secreted from dental pulp stem cells show multifaceted benefits for treating experimental temporomandibular joint osteoarthritis
• Author/Editor: Ogasawara, Naoko; Kano, Fumiya; Hashimoto, Noboru; Mori, Hiroki; Liu, Yao; Xia, L.; Sakamaki, T.; Hibi, H.; Iwamoto, Tsutomu; Tanaka, Eiji; Yamamoto, Akihito
• Author Heading: Ogasawara, Naoko; Kano, Fumiya; Hashimoto, Noboru; Mori, Hiroki; Liu, Yao; Xia, L.; Sakamaki, T.; Hibi, H.; Iwamoto, Tsutomu; Tanaka, Eiji; Yamamoto, Akihito
• Publication, Distribution, etc.: Osteoarthritis Research Society International|Elsevier Ltd.
• Publication Date: 2020-04-06
• Publication Date (W3CDTF): 2020-04-06
• Alternative Title: 歯髄幹細胞からの分泌因子は、顎関節変形性関節症の治療に多面的な効果を示す
• Periodical title: Osteoarthritis and Cartilage
• No. or year of volume/issue: 28 6
• Volume: 28
• Issue: 6
• Pages: 831-841
• ISSN (Periodical Title): ISSN : 1063-4584
• Degree grantor/type: 徳島大学
• Date Granted: 2020-09-10
• Dissertation Number: 甲第3444号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Dental pulp stem cell; Conditioned medium; Chondrocyte; Cartilage regeneration; Temporomandibular joint; Osteoarthritis
• Target Audience: 一般
• Note (General): Objective: Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease characterized by progressive cartilage degeneration, abnormal bone remodeling, and chronic pain. In this study, we aimed to investigate effective therapies to reverse or suppress TMJOA progression. Design: To this end, we performed intravenous administration of serum free conditioned media from human exfoliated deciduous teeth stem cells (SHED-CM) into a mechanical-stress induced murine TMJOA model. Results: SHED-CM administration markedly suppressed temporal muscle inflammation, and improved bone integrity and surface smoothness of the destroyed condylar cartilage. Moreover, SHED-CM treatment decreased the number of IL-1β, iNOS, and MMP-13 expressing chondrocytes, whereas it specifically increased PCNA-positive cells in the multipotent polymorphic cell layer. Notably, the numbers of TdT-mediated dUTP nick end labeling (TUNEL)-positive apoptotic chondrocytes in the SHED-CM treated condyles were significantly lower than in those treated with DMEM, whereas the proteoglycan positive area was restored to a level similar to that of the sham treated group, demonstrating that SHED-CM treatment regenerated the mechanical-stress injured condylar cartilage and subchondral bone. Secretome analysis revealed that SHED-CM contained multiple therapeutic factors that act in osteochondral regeneration. Conclusions: Our data demonstrated that SHED-CM treatment promoted the regeneration and repair of mechanical-stress induced mouse TMJOA. Our observations suggest that SHED-CM has potential to be a potent tissue-regenerating therapeutic agent for patients with severe TMJOA.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.joca.2020.03.010
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/115291/20200924151903109725/k3444_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/115291/20200924151903672086/k3444_review.pdf fulltext

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