Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration

• Material Type: 博士論文
• Title: Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration
• Author/Editor: Kawakita, Naoya; Toba, Hiroaki; Miyoshi, Keiko; Sakamoto, Shinichi; Matsumoto, Daisuke; Takashima, Mika; Aoyama, Mariko; Inoue, Seiya; Morimoto, Masami; Nishino, Takeshi; Takizawa, Hiromitsu; Tangoku, Akira
• Author Heading: Kawakita, Naoya; Toba, Hiroaki; Miyoshi, Keiko; Sakamoto, Shinichi; Matsumoto, Daisuke; Takashima, Mika; Aoyama, Mariko; Inoue, Seiya; Morimoto, Masami; Nishino, Takeshi; Takizawa, Hiromitsu; Tangoku, Akira
• Publication, Distribution, etc.: BioMed Central|Springer Nature
• Publication Date: 2020-10-02
• Publication Date (W3CDTF): 2020-10-02
• Alternative Title: マウスiPS細胞由来の気管支肺胞幹細胞は末梢気道上皮再生を促進する
• Periodical title: Stem Cell Research & Therapy
• No. or year of volume/issue: 11
• Volume: 11
• Pages: 430-
• ISSN (Periodical Title): ISSN : 1757-6512
• Degree grantor/type: 徳島大学
• Date Granted: 2020-11-26
• Dissertation Number: 甲第3466号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: stem cell; induced pluripotent stem cells; lung regeneration; bronchioloalveolar stem cell; Stem cell transplantation; Progenitor cells; Tissue regeneration
• Target Audience: 一般
• Note (General): Background: Bronchioalveolar stem cells (BASCs) located at the bronchioalveolar-duct junction (BADJ) are stem cells residing in alveoli and terminal bronchioles that can self-renew and differentiate into alveolar type (AT)-1 cells, AT-2 cells, club cells, and ciliated cells. Following terminal-bronchiole injury, BASCs increase in number and promote repair. However, whether BASCs can be differentiated from mouse-induced pluripotent stem cells (iPSCs) remains unreported, and the therapeutic potential of such cells is unclear. We therefore sought to differentiate BASCs from iPSCs and examine their potential for use in the treatment of epithelial injury in terminal bronchioles. Methods: BASCs were induced using a modified protocol for differentiating mouse iPSCs into AT-2 cells. Differentiated iPSCs were intratracheally transplanted into naphthalene-treated mice. The engraftment of BASCs into the BADJ and their subsequent ability to promote repair of injury to the airway epithelium were evaluated. Results: Flow cytometric analysis revealed that BASCs represented ~ 7% of the cells obtained. Additionally, ultrastructural analysis of these iPSC-derived BASCs via transmission electron microscopy showed that the cells containing secretory granules harboured microvilli, as well as small and immature lamellar body-like structures. When the differentiated iPSCs were intratracheally transplanted in naphthalene-induced airway epithelium injury, transplanted BASCs were found to be engrafted in the BADJ epithelium and alveolar spaces for 14 days after transplantation and to maintain the BASC phenotype. Notably, repair of the terminal-bronchiole epithelium was markedly promoted after transplantation of the differentiated iPSCs. Conclusions: Mouse iPSCs could be differentiated in vitro into cells that display a similar phenotype to BASCs. Given that the differentiated iPSCs promoted epithelial repair in the mouse model of naphthalene-induced airway epithelium injury, this method may serve as a basis for the development of treatments for terminal-bronchiole/alveolar-region disorders.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
• Related Material (DOI): 10.1186/s13287-020-01946-7
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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