Expression of fibroblast growth factor receptor1, -2c, and -3c transcripts in mouse molars after tooth eruption

• Material Type: 博士論文
• Title: Expression of fibroblast growth factor receptor1, -2c, and -3c transcripts in mouse molars after tooth eruption
• Author/Editor: Kano, Tsuyoshi; Morita, Tsuyoshi; Sumida, Kaori; Yumoto, Hiromichi; Baba, Otto
• Author Heading: Kano, Tsuyoshi; Morita, Tsuyoshi; Sumida, Kaori; Yumoto, Hiromichi; Baba, Otto
• Publication, Distribution, etc.: Springer Nature|Japanese Association of Anatomists
• Publication Date: 2021-01-12
• Publication Date (W3CDTF): 2021-01-12
• Alternative Title: マウス臼歯萌出後における線維芽細胞受容体 (Fgfr) 1, -2c, -3c 転写産物の発現
• Periodical title: Anatomical Science International
• No. or year of volume/issue: 96 2
• Volume: 96
• Issue: 2
• Pages: 301-309
• ISSN (Periodical Title): ISSN : 1447-6959; ISSN : 1447-073X
• Degree grantor/type: 徳島大学
• Date Granted: 2021-03-23
• Dissertation Number: 甲第3492号
• Degree Type: 博士（学術）
• Text Language Code: eng
• Subject Heading: Dentin formation; FGF receptor; Odontoblasts; Secondary dentin; Tooth eruption
• Target Audience: 一般
• Note (General): A previous study suggested that fibroblast growth factor (FGF) signaling plays an important role in dentin formation during tooth development. In this study, to examine dentin formation after tooth eruption involving secondary and tertiary dentin, we analyzed the expression patterns and expressing cells of Fgfr1, -2c, and -3c in mouse maxillary first molars (M1). Since it is difficult to recover the mRNAs from mineralized tissues, we tested methods for extraction after fixation and decalcification of teeth. We successfully obtained consistent results with quantitative real-time PCR (qPCR) using β-actin transcripts for validation. qPCR for Dentin sialo phosphoprotein (Dspp), Fgfr1, -2c, and -3c transcripts was performed on mice at ages of 2-20 weeks. The results showed that the highest expression levels of Dspp and Fgfr2c occurred at 2 weeks old followed by lower expression levels after 4 weeks old. However, the expression levels of Fgfr1 and Fgfr3c were constant throughout the experimental period. By in situ hybridization, Dspp, Fgfr1, and Fgfr3c transcripts were detected in odontoblasts at ages of 2 and 4 weeks. Also, Dspp and Fgfr1 transcripts were detected in odontoblasts facing reactionary dentin at 8 weeks old. These results suggest that FGF-FGFR signaling might be involved in the regulation of odontoblasts even after tooth eruption, including secondary and tertiary dentin formation. Moreover, our modified method for extracting mRNA from mineralized tissues after fixation and decalcification successfully produced consistent results.; By utilizing our modified RNA extraction method, we determined the expression of FGFRs after tooth eruption, which suggested the commitment of FGF signaling to the homeostasis of odontoblasts.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1007/s12565-020-00594-4
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/116058/20210615114736280890/k3492_abstract.pdf fulltext

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