Suppressive effects of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in endothelial cells

• Material Type: 博士論文
• Title: Suppressive effects of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in endothelial cells
• Author/Editor: Kondo-Kawai, Akari; Sakai, Tohru; Terao, Junji; Mukai, Rie
• Author Heading: Kondo-Kawai, Akari; Sakai, Tohru; Terao, Junji; Mukai, Rie
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2021-07-01
• Publication Date (W3CDTF): 2021-07-01
• Alternative Title: 血管内皮細胞における過酸化水素誘導のカベオリン-1リン酸化に対するケルセチンの抑制効果
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• No. or year of volume/issue: 69 1
• Volume: 69
• Issue: 1
• Pages: 28-36
• ISSN (Periodical Title): ISSN : 0912-0009; ISSN : 1880-5086
• Degree grantor/type: 徳島大学
• Date Granted: 2021-03-23
• Dissertation Number: 甲第3510号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: quercetin; caveolae; caveolin-1; hydrogen peroxide; vascular endothelium
• Target Audience: 一般
• Note (General): Caveolin-1 is a major protein of the caveolae structure in vascular endothelial cell membrane. Phosphorylation of caveolin-1 is one of the initial events leading to exacerbation of vascular permeability caused by oxidative stress. Although quercetin is known to be an anti-atherosclerosis factor that acts as a dietary antioxidant, little is known about its role in the regulation of caveolin-1 phosphorylation. In this study, we investigated the inhibitory effect of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in human umbilical vein endothelial cells. Quercetin inhibited caveolin-1 phosphorylation in cells pretreated with quercetin for 24 h and then exposed to hydrogen peroxide. However, quercetin 3-O-β-glucuronide, a conjugated metabolite of quercetin, did not exert this inhibitory effect. Exposure to hydrogen peroxide increased vascular permeability and reduced mRNA expression of the intercellular adhesion protein, vascular endothelial cadherin (VE-cadherin). By contrast, pretreatment with quercetin suppressed the increase in vascular permeability and decreased VE-cadherin expression. These results indicate that deconjugated quercetin can play a role in the prevention of altered vascular permeability under oxidative stress by suppressing caveolin-1 phosphorylation. Thus, dietary quercetin may be beneficial for the maintenance of endothelial cell function.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2020 Society for Free Radical Research Japan This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License. https://creativecommons.org/licenses/by-nc-nd/4.0/
• Related Material (DOI): 10.3164/jcbn.20-190
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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