Dynamics of D-amino acid oxidase in kidney epithelial cells under amino acid starvation

• Material Type: 博士論文
• Title: Dynamics of D-amino acid oxidase in kidney epithelial cells under amino acid starvation
• Author/Editor: Sogabe, Hirofumi; Shishido, Yuji; Miyazaki, Hayato; Kim, Soo Hyeon; Rachadech, Wanitcha; Fukui, Kiyoshi
• Author Heading: Sogabe, Hirofumi; Shishido, Yuji; Miyazaki, Hayato; Kim, Soo Hyeon; Rachadech, Wanitcha; Fukui, Kiyoshi
• Publication, Distribution, etc.: Oxford University Press
• Publication Date: 2021-03-16
• Publication Date (W3CDTF): 2021-03-16
• Alternative Title: アミノ酸飢餓下の腎臓上皮細胞におけるD-アミノ酸酸化酵素の動態; Dynamics of DAO protein in kidney cells under AA starvation
• Periodical title: The Journal of Biochemistry
• No. or year of volume/issue: 170 1
• Volume: 170
• Issue: 1
• Pages: 119-129
• ISSN (Periodical Title): ISSN : 1756-2651; ISSN : 0021-924X
• Degree grantor/type: 徳島大学
• Date Granted: 2021-04-22
• Dissertation Number: 甲第3528号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: D-amino acid oxidase; Kidney epithelial cell; Amino acid starvation; Peroxisome; Autophagy
• Target Audience: 一般
• Note (General): D-amino acid oxidase (DAO) is a flavoenzyme catalyzing the oxidation of D-amino acid (AA)s.In the kidney, its expression is detected in proximal tubules, and DAO is considered to play a role in the conversion of D-form AAs to α-keto acids. LLC-PK1 cells, a pig renal proximal tubule cell line, were used to elucidate the regulation of DAO protein synthesis and degradation. In this study, we showed that trypsinization of LLC-PK1 cells in culture system rapidly reduced the intracellular DAO protein level to approximately 33.9% of that before treatment, even within 30 min. Furthermore, we observed that the DAO protein level was decreased when LLC-PK1 cells were subjected to AA starvation. To determine the degradation pathway, we treated the cells with chloroquine and MG132. DAO degradation was found to be inhibited by chloroquine, but not by MG132 treatment. We next examined whether or not DAO was degraded by autophagy. We found that AA starvation led to an increased accumulation of LC3-II, suggesting that DAO protein is degraded by autophagy due to AA starvation conditions. Furthermore, treatment with cycloheximide inhibited DAO protein degradation. Taken together, DAO protein is degraded by autophagy under starvation. The present study revealed the potential dynamics of DAO correlated with renal pathophysiology.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1093/jb/mvab029
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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