Applying Probe Electrospray Ionization Mass Spectrometry to Cytological Diagnosis : A Preliminary Study by Using Cultured Lung Cancer Cells

• Material Type: 博士論文
• Title: Applying Probe Electrospray Ionization Mass Spectrometry to Cytological Diagnosis : A Preliminary Study by Using Cultured Lung Cancer Cells
• Author/Editor: Morimoto, Yuki; Oya, Takeshi; Ichimura-Shimizu, Mayuko; Matsumoto, Minoru; Ogawa, Hirohisa; Kobayashi, Tomoko; Sumida, Satoshi; Kakimoto, Takumi; Yamashita, Michiko; Cheng, Chunmei; Tsuneyama, Koichi
• Author Heading: Morimoto, Yuki; Oya, Takeshi; Ichimura-Shimizu, Mayuko; Matsumoto, Minoru; Ogawa, Hirohisa; Kobayashi, Tomoko; Sumida, Satoshi; Kakimoto, Takumi; Yamashita, Michiko; Cheng, Chunmei; Tsuneyama, Koichi
• Publication, Distribution, etc.: Karger
• Publication Date: 2021-06-07
• Publication Date (W3CDTF): 2021-06-07
• Alternative Title: 細胞診における探針エレクトロスプレーイオン化質量分析の有用性 : 肺癌培養細胞での検討; PESI-MS for Diagnostic Cytology
• Periodical title: Acta Cytologica
• No. or year of volume/issue: 65 5
• Volume: 65
• Issue: 5
• Pages: 430-439
• ISSN (Periodical Title): ISSN : 0001-5547; ISSN : 1938-2650
• Degree grantor/type: 徳島大学
• Date Granted: 2021-09-09
• Dissertation Number: 甲第3535号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Mass spectrometry; Rapid cytological diagnosis; Probe electrospray ionization mass spectrometry; Lung cytopathology
• Target Audience: 一般
• Note (General): Objectives: Cytology and histology are 2 indispensable diagnostic tools for cancer diagnosis, which are rapidly increasing in importance with aging populations. We applied mass spectrometry (MS) as a rapid approach for swiftly acquiring nonmorphological information of interested cells. Conventional MS, which primarily rely on promoting ionization by pre-applying a matrix to cells, has the drawback of time-consuming both on data acquisition and analysis. As an emerging method, probe electrospray ionization-MS (PESI-MS) with a dedicated probe is capable to pierce sample and measure specimen in small amounts, either liquid or solid, without the requirement for sample pretreatment. Furthermore, PESI-MS is timesaving compared to the conventional MS. Herein, we investigated the capability of PESI-MS to characterize the cell types derived from the respiratory tract of human tissues. Study Design: PESI-MS analyses with DPiMS-2020 were performed on various type of cultured cells including 5 lung squamous cell carcinomas, 5 lung adenocarcinomas, 5 small-cell carcinomas, 4 malignant mesotheliomas, and 2 normal controls. Results: Several characteristic peaks were detected at around m/z 200 and 800 that were common in all samples. As expected, partial least squares-discriminant analysis of PESI-MS data distinguished the cancer cell types from normal control cells. Moreover, distinct clusters divided squamous cell carcinoma from adenocarcinoma. Conclusion: PESI-MS presented a promising potential as a novel diagnostic modality for swiftly acquiring specific cytological information.
• Access Restrictions: インターネット公開
• Rights (production): © 2021 S. Karger AG, Basel
• Related Material (DOI): 10.1159/000516639
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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