Protective effects of resveratrol against 5-fluorouracil-induced oxidative stress and inflammatory responses in human keratinocytes

• Material Type: 博士論文
• Title: Protective effects of resveratrol against 5-fluorouracil-induced oxidative stress and inflammatory responses in human keratinocytes
• Author/Editor: Chen, Shu; Tamaki, Naofumi; Kudo, Yasusei; Tsunematsu, Takaaki; Miki, Kaname; Ishimaru, Naozumi; Ito, Hiro-O
• Author Heading: Chen, Shu; Tamaki, Naofumi; Kudo, Yasusei; Tsunematsu, Takaaki; Miki, Kaname; Ishimaru, Naozumi; Ito, Hiro-O
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2021-11-01
• Publication Date (W3CDTF): 2021-11-01
• Alternative Title: ヒト角化上皮細胞における5-FU誘発性酸化ストレスおよび炎症応答に対するresveratrolの保護効果
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• No. or year of volume/issue: 69 3
• Volume: 69
• Issue: 3
• Pages: 238-246
• ISSN (Periodical Title): ISSN : 0912-0009; ISSN : 1880-5086
• Degree grantor/type: 徳島大学
• Date Granted: 2021-09-09
• Dissertation Number: 甲第3541号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: oxidative stress; inflammation; oral mucositis; antioxidant; human normal keratinocyte; resveratrol
• Target Audience: 一般
• Note (General): Although 5-fluorouracil (5-FU) is currently used as an anti-cancer chemotherapy, adverse effects such as oral mucositis potentially limit its clinical application. Additionally, the prevention of 5-FU-induced side effects are scarce. Resveratrol is known to decrease oxidative damage and inflammation. In this study, we examined the protective effects of resveratrol on 5-FU-induced oxidative stress and inflammatory responses in normal human keratinocytes (HaCaT cell) as in vitro oral mucositis model. HaCaT cells were exposed to 5-FU and simultaneously treated with resveratrol. The effects of resveratrol on 5-FU-induced cytotoxicity were evaluated using cell viability assay. The production of reactive oxygen species (ROS) was measured using a fluorescence spectrophotometer. The effects of resveratrol on nuclear factor erythroid 2-related factor 2 (Nrf2), silent information regulator transcript-1 (SIRT-1), and nuclear factor kappa B (NF-κB) signaling and inflammatory cytokine expression were examined. Resveratrol suppressed 5-FU-induced overproduction of ROS by upregulating anti-oxidant defense genes through Nrf2 activation and SIRT-1 expression. Concerning inflammatory responses, resveratrol suppressed the 5-FU-induced expression of pro-inflammatory cytokines via NF-κB nuclear translocation. Conversely, N-acetylcysteine reduced ROS levels without affecting the expression of pro-inflammatory cytokines. Resveratrol might be useful for preventing 5-FU-induced adverse effects by activating anti-oxidant and anti-inflammatory responses.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
• Related Material (DOI): 10.3164/jcbn.21-23
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/116380/20211005113908490439/k3541_abstract.pdf fulltext

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