Peripherally Administered Botulinum Toxin Type a Localizes Bilaterally in Trigeminal Ganglia of Animal Model

• Material Type: 博士論文
• Title: Peripherally Administered Botulinum Toxin Type a Localizes Bilaterally in Trigeminal Ganglia of Animal Model
• Author/Editor: Waskitho, Arief; Yamamoto, Yumiko; Raman, Swarnalakshmi; Kano, Fumiya; Yan, Huijiao; Raju, Resmi; Afroz, Shaista; Morita, Tsuyoshi; Ikutame, Daisuke; Okura, Kazuo; Oshima, Masamitsu; Yamamoto, Akihito; Baba, Otto; Matsuka, Yoshizo
• Author Heading: Waskitho, Arief; Yamamoto, Yumiko; Raman, Swarnalakshmi; Kano, Fumiya; Yan, Huijiao; Raju, Resmi; Afroz, Shaista; Morita, Tsuyoshi; Ikutame, Daisuke; Okura, Kazuo; Oshima, Masamitsu; Yamamoto, Akihito; Baba, Otto; Matsuka, Yoshizo
• Publication, Distribution, etc.: MDPI
• Publication Date: 2021-10-05
• Publication Date (W3CDTF): 2021-10-05
• Alternative Title: 片側末梢投与されたA型ボツリヌス毒素は動物モデルにおいて両側三叉神経節に局在する
• Periodical title: Toxins
• No. or year of volume/issue: 13 10
• Volume: 13
• Issue: 10
• Pages: 704-
• ISSN (Periodical Title): ISSN : 2072-6651
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-23
• Dissertation Number: 甲第3609号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Botulinum toxin; trigeminal ganglion; neuropathic pain
• Target Audience: 一般
• Note (General): Peripheral nerve injury leads to sensory ganglion hyperexcitation, which increases neurotransmitter release and neuropathic pain. Botulinum toxin type A (BoNT/A) regulates pain transmission by reducing neurotransmitter release, thereby attenuating neuropathic pain. Despite multiple studies on the use of BoNT/A for managing neuropathic pain in the orofacial region, its exact mechanism of transport remains unclear. In this study, we investigated the effects of BoNT/A in managing neuropathic pain in two different animal models and its transport mechanism in the trigeminal nerve. Intraperitoneal administration of cisplatin induced bilateral neuropathic pain in the orofacial region, reducing the head withdrawal threshold to mechanical stimulation. Unilateral infraorbital nerve constriction (IONC) also reduced the ipsilateral head withdrawal threshold to mechanical stimulation. Unilateral peripheral administration of BoNT/A to the rat whisker pad attenuated cisplatin-induced pain behavior bilaterally. Furthermore, contralateral peripheral administration of BoNT/A attenuated neuropathy-induced behavior caused by IONC. We also noted the presence of BoNT/A in the blood using the mouse bioassay. In addition, the Alexa Fluor-488-labeled C-terminal half of the heavy chain of BoNT/A (BoNT/A-Hc) was localized in the neurons of the bilateral trigeminal ganglia following its unilateral administration. These findings suggest that axonal and hematogenous transport are involved in the therapeutic effects of peripherally administered BoNT/A in the orofacial region.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• Related Material (DOI): 10.3390/toxins13100704
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/116383/20220408115207509736/k3609_abstract.pdf fulltext

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