Additional effects of simultaneous treatment with C14-Cblin and celastrol on the clinorotation-induced rat L6 myotube atrophy

• Material Type: 博士論文
• Title: Additional effects of simultaneous treatment with C14-Cblin and celastrol on the clinorotation-induced rat L6 myotube atrophy
• Author/Editor: Kitahata, Kanako; Uchida, Takayuki; Taniguchi, Runa; Kato, Ayano; Sugiura, Kosuke; Sakakibara, Iori; Oarada, Motoko; Fukawa, Tomoya; Junsoo, Park; Inho, Choi; Nikawa, Takeshi
• Author Heading: Kitahata, Kanako; Uchida, Takayuki; Taniguchi, Runa; Kato, Ayano; Sugiura, Kosuke; Sakakibara, Iori; Oarada, Motoko; Fukawa, Tomoya; Junsoo, Park; Inho, Choi; Nikawa, Takeshi
• Publication, Distribution, etc.: Tokushima University Faculty of Medicine
• Publication Date: 2022-02
• Publication Date (W3CDTF): 2022-02
• Alternative Title: 3D-clinorotationによるラットL6筋管萎縮に対するC14-Cblin及びcelastrolの同時投与による相加効果; Effect of Cblin and celastrol on muscle atrophy
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 69 1-2
• Volume: 69
• Issue: 1-2
• Pages: 127-134
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-10
• Dissertation Number: 甲第3584号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: C14-Cblin; celastrol; Cbl-b; 筋萎縮; 酸化ストレス; myotube atrophy; muscle atrophy-associated ubiquitin ligases; oxidative stress
• Target Audience: 一般
• Note (General): Two novel reagents, N-myristoylated Cbl-b inhibitory peptide (C14-Cblin) and celastrol, a quinone methide triterpene, are reported to be effective in preventing myotube atrophy. The combined effects of C14-Cblin and celastrol on rat L6 myotubes atrophy induced by 3D-clinorotation, a simulated microgravity model, was investigated in the present study. We first examined their effects on expression in atrogenes. Increase in MAFbx1/atrogin-1 and MuRF-1 by 3D-clinorotation was significantly suppressed by treatment with C14-Cblin or celastrol, but there was no additive effect of simultaneous treatment. However, celastrol significantly suppressed the upregulation of Cbl-b and HSP70 by 3D-clinorotation. Whereas 3D-clinorotation decreased the protein level of IRS-1 in L6 myotubes, C14-Cblin and celastrol inhibited the degradation of IRS-1. C14-Cblin and celastrol promoted the phosphorylation of FOXO3a even in microgravity condition. Simultaneous administration of C14-Cblin and celastrol had shown little additive effect in reversing the impairment of IGF-1 signaling by 3D-clinorotation. While 3D-clinorotation-induced marked oxidative stress in L6 myotubes, celastrol suppressed 3D-clinorotation-induced ROS production. Finally, the C14-Cblin and celastrol-treated groups were inhibited decrease in L6 myotube diameter and increased the protein content of slow-twitch MyHC cultured under 3D-clinorotation. The simultaneous treatment of C14-Cblin and celastrol additively prevented 3D-clinorotation-induced myotube atrophy than single treatment.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.69.127
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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