Intrathymic Plasmablasts Are Affected in Patients With Myasthenia Gravis With Active Disease

• Material Type: 博士論文
• Title: Intrathymic Plasmablasts Are Affected in Patients With Myasthenia Gravis With Active Disease
• Author/Editor: Yamamoto, Yohei; Matsui, Naoko; Uzawa, Akiyuki; Ozawa, Yukiko; Kanai, Tetsuya; Oda, Fumiko; Kondo, Hiroyuki; Ohigashi, Izumi; Takizawa, Hiromitsu; Kondo, Kazuya; Sugano, Mikio; Kitaichi, Takashi; Hata, Hiroki; Kaji, Ryuji; Kuwabara, Satoshi; Yamamura, Takashi; Izumi, Yuishin
• Author Heading: Yamamoto, Yohei; Matsui, Naoko; Uzawa, Akiyuki; Ozawa, Yukiko; Kanai, Tetsuya; Oda, Fumiko; Kondo, Hiroyuki; Ohigashi, Izumi; Takizawa, Hiromitsu; Kondo, Kazuya; Sugano, Mikio; Kitaichi, Takashi; Hata, Hiroki; Kaji, Ryuji; Kuwabara, Satoshi; Yamamura, Takashi; Izumi, Yuishin
• Publication, Distribution, etc.: Wolters Kluwer|American Academy of Neurology
• Publication Date: 2021-09-24
• Publication Date (W3CDTF): 2021-09-24
• Alternative Title: 疾患活動性の高い重症筋無力症患者の胸腺ではプラズマブラストが増加している
• Periodical title: Neurology : Neuroimmunology & Neuroinflammation
• No. or year of volume/issue: 8 6
• Volume: 8
• Issue: 6
• Pages: e1087-
• ISSN (Periodical Title): ISSN : 2332-7812
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-04
• Dissertation Number: 甲第3581号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Myasthenia Gravis; Thymectomy; Plasmablast; B cell; Tfh cell
• Target Audience: 一般
• Note (General): Background and Objectives To investigate intrathymic B lymphopoiesis in patients with myasthenia gravis (MG) and explore thymus pathology associated with clinical impact. Methods Thymic lymphocytes from 15 young patients without MG, 22 adult patients without MG, 14 patients with MG without thymoma, and 11 patients with MG with thymoma were subjected to flow cytometry analysis of T follicular helper (Tfh), naive B, memory B, plasmablasts, CD19+B220high thymic B cells, B-cell activating factor receptor, and C-X-C chemokine receptor 5 (CXCR5). Peripheral blood mononuclear cells of 16 healthy subjects and 21 untreated patients with MG were also analyzed. Immunologic values were compared, and correlations between relevant values and clinical parameters were evaluated. Results The frequencies of circulating and intrathymic plasmablasts were significantly higher in patients with MG than controls. On the other hand, the frequency of CD19+B220high thymic B cells was not increased in MG thymus. We observed a significant increase in CXCR5 expression on plasmablasts in MG thymus and an increased frequency of intrathymic plasmablasts that was correlated with preoperative disease activity. The frequency of intrathymic Tfh cells was significantly lower in patients who received immunosuppressive (IS) therapy than those without IS therapy. However, there was no significant difference in the frequency of intrathymic plasmablasts irrespective of IS therapy. Discussion Our findings confirmed a correlation between increased frequency of intrathymic plasmablasts and disease activity before thymectomy. We postulate that activated intrathymic plasmablasts endow pathogenic capacity in MG.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND), which permits downloading and sharing the work provided it is properly cited ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ). The work cannot be changed in any way or used commercially without permission from the journal.
• Related Material (DOI): 10.1212/NXI.0000000000001087
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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