Dentin-Pulp Complex Tissue Regeneration via Three-Dimensional Cell Sheet Layering

• Material Type: 博士論文
• Title: Dentin-Pulp Complex Tissue Regeneration via Three-Dimensional Cell Sheet Layering
• Author/Editor: Yan, Huijiao; Oshima, Masamitsu; Raju, Resmi; Raman, Swarnalakshmi; Sekine, Kazumitsu; Waskitho, Arief; Inoue, Miho; Inoue, Masahisa; Baba, Otto; Morita, Tsuyoshi; Miyagi, Mayu; Matsuka, Yoshizo
• Author Heading: Yan, Huijiao; Oshima, Masamitsu; Raju, Resmi; Raman, Swarnalakshmi; Sekine, Kazumitsu; Waskitho, Arief; Inoue, Miho; Inoue, Masahisa; Baba, Otto; Morita, Tsuyoshi; Miyagi, Mayu; Matsuka, Yoshizo
• Publication, Distribution, etc.: Mary Ann Liebert, Inc
• Publication Date: 2021-10-15
• Publication Date (W3CDTF): 2021-10-15
• Alternative Title: 積層細胞シート化技術を用いた象牙質 : 歯髄複合組織の再生; Dentin-pulp regeneration by 3D layered cell sheet
• Periodical title: Tissue Engineering Part C: Methods
• No. or year of volume/issue: 27 10
• Volume: 27
• Issue: 10
• Pages: 559-570
• ISSN (Periodical Title): ISSN : 1937-3384; ISSN : 1937-3392
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-23
• Dissertation Number: 甲第3610号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Dentin-pulp complex; cell sheet; regeneration; dental pulp cells; tissue engineering
• Target Audience: 一般
• Note (General): The dentin-pulp complex is a unique structure in teeth that contains both hard and soft tissues. Generally, deep caries and trauma cause damage to the dentin-pulp complex, and if left untreated, this damage will progress to irreversible pulpitis. The aim of this study was to fabricate a layered cell sheet composed of rat dental pulp (DP) cells and odontogenic differentiation of pulp (OD) cells and to investigate the ability to regenerate the dentin-pulp complex in a scaffold tooth. We fabricated two single cell sheets composed of DP cells (DP cell sheet) or OD cells (OD cell sheet) and a layered cell sheet made by layering both cells. The characteristics of the fabricated cell sheets were analyzed using light microscopy, scanning electron microscopy (SEM), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC). Furthermore, the cell sheets were transplanted into the subrenal capsule of immunocompromised mice for 8 weeks. Following this, the regenerative capacity to form dentin-like tissue was evaluated using micro-computed tomography (Micro-CT), HE staining, and IHC. The findings of SEM and IHC confirmed that layered cell sheets fabricated by stacking OD cells and DP cells maintained their cytological characteristics. Micro-CT of layered cell sheet transplants revealed a mineralized capping of the access cavity in the crown area, similar to that of natural dentin. In contrast, the OD cell sheet group demonstrated the formation of irregular fragments of mineralized tissue in the pulp cavity, and the DP cell sheet did not develop any hard tissue. Moreover, bone volume/tissue volume (BV/TV) showed a significant increase in hard tissue formation in the layered cell sheet group compared to that in the single cell sheet group (p<0.05). HE staining also showed a combination of soft and hard tissue formation in the layered cell sheet group. Furthermore, IHC confirmed that the dentin-like tissue generated from the layered cell sheet expressed characteristic markers of dentin but not bone equivalent to that of a natural tooth. In conclusion, this study demonstrates the feasibility of regenerating dentin-pulp complex using a bioengineered tissue designed to simulate the anatomical structure.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is the accepted version of the following article: Dentin-Pulp Complex Tissue Regeneration via Three-Dimensional Cell Sheet Layering; Huijiao Yan, Masamitsu Oshima, Resmi Raju, Swarnalakshmi Raman, Kazumitsu Sekine, Arief Waskitho, Miho Inoue, Masahisa Inoue, Otto Baba, Tsuyoshi Morita, Mayu Miyagi, and Yoshizo Matsuka; Tissue Engineering Part C: Methods 2021 27:10, 559-570, which has now been formally published in final form at Tissue Engineering Part C: Methods at https://doi.org/10.1089/ten.tec.2021.0171. This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers’ self-archiving terms and conditions.
• Related Material (DOI): 10.1089/ten.tec.2021.0171
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

Table of Contents

http://repo.lib.tokushima-u.ac.jp/files/public/11/117029/20220513151705867431/k3610_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117029/20220513151705999894/k3610_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117029/20220603100726743180/k3610_fulltext.pdf fulltext