Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids

• Material Type: 博士論文
• Title: Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids
• Author/Editor: Nishimura, Ryosuke; Kato, Kagayaki; Saida, Misako; Kamei, Yasuhiro; Takeda, Masahiro; Miyoshi, Hiromi; Yamagata, Yutaka; Amano, Yu; Yonemura, Shigenobu
• Author Heading: Nishimura, Ryosuke; Kato, Kagayaki; Saida, Misako; Kamei, Yasuhiro; Takeda, Masahiro; Miyoshi, Hiromi; Yamagata, Yutaka; Amano, Yu; Yonemura, Shigenobu
• Publication, Distribution, etc.: Japan Society for Cell Biology
• Publication Date: 2022-06-22
• Publication Date (W3CDTF): 2022-06-22
• Alternative Title: αカテニンの適切な張力感受性は上皮スフェロイドの球形への形態形成を確実にする; Tension-sensitivity of α-catenin on morphogenesis
• Periodical title: Cell Structure and Function
• No. or year of volume/issue: 47 2
• Volume: 47
• Issue: 2
• Pages: 55-73
• ISSN (Periodical Title): ISSN : 1347-3700; ISSN : 0386-7196
• Degree grantor/type: 徳島大学
• Date Granted: 2022-07-28
• Dissertation Number: 甲第3644号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: αカテニン; ビンキュリン; 細胞間接着; 上皮形態形成; メカノトランスダクション; α-catenin; vinculin; adherens junction; morphogenesis; mechanotransduction
• Target Audience: 一般
• Note (General): The adherens junction (AJ) is an actin filament-anchoring junction. It plays a central role in epithelial morphogenesis through cadherin-based recognition and adhesion among cells. The stability and plasticity of AJs are required for the morphogenesis. An actin-binding α-catenin is an essential component of the cadherin-catenin complex and functions as a tension transducer that changes its conformation and induces AJ development in response to tension. Despite much progress in understanding molecular mechanisms of tension sensitivity of α-catenin, its significance on epithelial morphogenesis is still unknown. Here we show that the tension sensitivity of α-catenin is essential for epithelial cells to form round spheroids through proper multicellular rearrangement. Using a novel in vitro suspension culture model, we found that epithelial cells form round spheroids even from rectangular-shaped cell masses with high aspect ratios without using high tension and that increased tension sensitivity of α-catenin affected this morphogenesis. Analyses of AJ formation and cellular tracking during rounding morphogenesis showed cellular rearrangement, probably through AJ remodeling. The rearrangement occurs at the cell mass level, but not single-cell level. Hypersensitive α-catenin mutant-expressing cells did not show cellular rearrangement at the cell mass level, suggesting that the appropriate tension sensitivity of α-catenin is crucial for the coordinated round morphogenesis.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.
• Related Material (DOI): 10.1247/csf.22014
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/117376/20220824092436700010/k3644_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117376/20220824092436968490/k3644_review.pdf fulltext

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